体外培养的完整大鼠肝线粒体中，酰基辅酶A添加方式对肉碱存在时酰基肉碱初始形成速率的影响。
Effects of the mode of addition of acyl-CoA on the initial rate of formation of acylcarnitine in the presence of carnitine by intact rat liver mitochondria in vitro.
作者信息
Zammit V A
出版信息
Biochem J. 1985 Jul 1;229(1):273-5. doi: 10.1042/bj2290273.
Abstract
Time courses for the formation of palmitoylcarnitine from palmitoyl-CoA and carnitine, catalysed by the overt activity of carnitine palmitoyltransferase (CPT I) in rat liver mitochondria, were obtained. Significant initial non-linearity was observed only when reactions were started by addition of a concentrated solution of palmitoyl-CoA (4mM, to give a final concentration of 100 microM) uncomplexed to albumin. Minimal effects were observed when the reactions were started by addition of palmitoyl-CoA-albumin mixtures, even though the final palmitoyl-CoA/albumin molar ratios in the assay medium were identical in the two sets of experiments.
摘要
获得了在大鼠肝脏线粒体中肉碱棕榈酰转移酶（CPT I）的明显活性催化下，由棕榈酰辅酶A和肉碱形成棕榈酰肉碱的时间进程。仅当通过添加未与白蛋白复合的棕榈酰辅酶A浓溶液（4mM，最终浓度为100μM）启动反应时，才观察到显著的初始非线性。当通过添加棕榈酰辅酶A - 白蛋白混合物启动反应时，观察到的影响最小，尽管两组实验中测定介质中最终的棕榈酰辅酶A/白蛋白摩尔比相同。
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本文引用的文献
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