Braginskaia P S, Shilova S V, Chaĭkovskaia S M
Antibiotiki. 1977 Mar;22(3):208-11.
Optimal conditions for determination of microbial contamination of drugs were studied on artificially contaminated powders and tablets of phenoxymethylpenicillin, ampicillin, oxacillin and dicloxacillin. The method of membrane filtration was the best for determination of the microbial contamination of the powders. However, it was not possible to wash out completely the antibiotic from the membrane filter. To prevent this it was necessary to add penicillinase into the nutrient medium onto which the filter was put for providing the microbial growth. For determination of microbial contamination of tablets direct plating of 3 per cent suspension of the tablet mass onto the surface of the nutrient medium with penicillinase was the best.
在人工污染的苯氧甲基青霉素、氨苄西林、苯唑西林和双氯西林粉末及片剂上，研究了测定药物微生物污染的最佳条件。膜过滤法是测定粉末微生物污染的最佳方法。然而，无法将抗生素从膜过滤器中完全冲洗掉。为防止这种情况，有必要在放置过滤器以促进微生物生长的营养培养基中添加青霉素酶。对于片剂微生物污染的测定，将3%片剂混悬液直接接种在含青霉素酶的营养培养基表面是最佳方法。
