淋病奈瑟菌1型和4型的放射性标记及大分子合成
Radiolabeling of and macromolecular syntheses in Neisseria gonorrhoeae types 1 and 4.
作者信息
Lim D V, James A N, Williams R P
出版信息
Appl Environ Microbiol. 1977 Feb;33(2):328-33. doi: 10.1128/aem.33.2.328-333.1977.
Abstract
Type 1 and type 4 cells of Neisseria gonorrhoeae were grown in a liquid medium that maintained colony type for several hours. Organisms from both types of colonies incorporated labeled glucose, as well as nucleic acid and protein precursors, into specific cellular fractions during growth in the medium. Ribonucleic acid and protein were synthesized actively by the bacteria, with incorporation of label chased by the addition of unlabeled precursors. The method can be used to label type 1 and type 4 cells of gonococci with specific isotopes for studies of metabolic and biochemical changes.
摘要
淋病奈瑟菌1型和4型细胞在一种能维持菌落类型数小时的液体培养基中生长。在培养基中生长期间，来自这两种菌落类型的微生物将标记的葡萄糖以及核酸和蛋白质前体掺入特定的细胞组分中。细菌积极合成核糖核酸和蛋白质，添加未标记的前体后标记物的掺入量会减少。该方法可用于用特定同位素标记淋病奈瑟菌的1型和4型细胞，以研究代谢和生化变化。
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