Dubé F, Schmidt T, Johnson C H, Epel D
Cell. 1985 Mar;40(3):657-66. doi: 10.1016/0092-8674(85)90214-4.
The intracellular pH (pHi) rises 0.3-0.5 units after fertilization of sea urchin eggs, and this and previous work show this pHi change is necessary for initiating the developmental processes leading to cell division. The experiments described here reveal that while the elevated pHi is permanently required for a normal early development, lowering pHi of embryos after fertilization affects different processes to different extents. Protein synthesis gradually becomes less sensitive to pHi. Karyokinesis proceeds to completion under a low pHi, but is retarded, while cytokinesis is always impaired. These results indicate a hierarchy of requirements for high pHi during early development of sea urchin embryos, with protein synthesis, karyokinesis, and cytokinesis showing, respectively, increasing requirements for an elevated pHi.
海胆卵受精后，细胞内pH值（pHi）升高0.3 - 0.5个单位，本研究及之前的工作表明，这种pHi变化是启动导致细胞分裂的发育过程所必需的。此处描述的实验表明，虽然正常早期发育始终需要升高的pHi，但受精后降低胚胎的pHi对不同过程的影响程度不同。蛋白质合成对pHi的敏感性逐渐降低。在低pHi条件下核分裂可进行至完成，但会受到阻碍，而胞质分裂总是受损。这些结果表明，海胆胚胎早期发育过程中对高pHi的需求存在层次结构，蛋白质合成、核分裂和胞质分裂对升高的pHi的需求分别增加。
