Kool H J, Kuper F, van Haeringen H, Koeman J H
Food Chem Toxicol. 1985 Jan;23(1):79-85. doi: 10.1016/0278-6915(85)90224-8.
The carcinogenicity in male and female Wistar SSP TOX rats of organic drinking-water concentrates that are positive in the Ames test was studied at three doses. The organic mutagenic concentrates were prepared weekly from drinking-water from one location in The Netherlands by adsorption onto XAD-4/8 resins and elution with dimethylsulphoxide. The organic concentrates in dimethylsulphoxide were mixed with non-mutagenic drinking-water before exposure of the rats. Dose levels were based on multiples of expected human exposure levels. For the calculation the average human daily intake of drinking-water was taken as 2 litres for a body weight of 70 kg. There was no significant increase in tumour induction when male Wistar SSP TOX rats were exposed for 106 wk to 4.5, 14 or 40 times the expected human exposure level and females to 7,22 or 68 times the human level. The development and types of tumours were similar in the treated and control groups. The numbers of animals with tumours and of animals that died as a result of tumours in the exposed groups did not differ significantly from those in the control groups. These results suggest that these organic mutagenic drinking-water concentrates did not contain very potent carcinogens in effective concentrations.
对Ames试验呈阳性的有机饮用水浓缩物在雄性和雌性Wistar SSP TOX大鼠中的致癌性进行了三种剂量的研究。有机诱变浓缩物每周从荷兰一个地点的饮用水中制备，通过吸附到XAD - 4/8树脂上并用二甲基亚砜洗脱。在大鼠接触前，将二甲基亚砜中的有机浓缩物与无诱变作用的饮用水混合。剂量水平基于预期人类接触水平的倍数。计算时，对于体重70千克的人，平均每日饮用水摄入量取2升。当雄性Wistar SSP TOX大鼠暴露106周于预期人类接触水平的4.5倍、14倍或40倍，雌性大鼠暴露于人类水平的7倍、22倍或68倍时，肿瘤诱发率没有显著增加。治疗组和对照组的肿瘤发生情况及类型相似。暴露组中患肿瘤的动物数量和因肿瘤死亡的动物数量与对照组相比无显著差异。这些结果表明，这些有机诱变饮用水浓缩物中不含有有效浓度的强效致癌物。
