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茶叶（茶树）中茶氨酸和表没食子儿茶素-3'-甲醚含量丰富的机制的多组学分析

Multi-omics analysis of the mechanisms of abundant theacrine and EGCG3"Me in tea (Camellia sinensis).

作者信息

Zhu Yanyu, Gu Mengya, Yu Wentao, Liao Longhua, Gao Shuilian, Wang Shuyan, Lin Hongzheng, Gui Wenjing, Zhou Youliang, Chen Zhiming, Zeng Jingde, Ye Naixing

机构信息

College of Horticulture-Key Laboratory of Tea Science at Universities in Fujian, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Technology Center of Fuzhou Customs District PR China, Fuzhou, 350001, Fujian, China.

出版信息

BMC Plant Biol. 2025 May 19;25(1):663. doi: 10.1186/s12870-025-06691-8.

DOI:10.1186/s12870-025-06691-8
PMID:40389844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12087082/
Abstract

Theacrine and epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me) are notable secondary metabolites in tea (Camellia sinensis), celebrated for their unique flavors and significant health effects. Theacrine has a mild effect on nerve stimulation, while EGCG3"Me exhibits better stability, higher oral bioavailability and stronger biological activity. However, tea plant varieties naturally rich in both theacrine and EGCG3"Me are rare. This study unveils a unique tea variety 'Anxi kucha', which is abundant in both theacrine and EGCG3"Me. Through integrated transcriptome-proteome-metabolome analysis, SAMS3, APRT1, IMPDH, and TCS1 were identified as critical enzymes for theacrine synthesis; while CHI1, CHI2, FLS2 and LAR1 were key for EGCG3"Me synthesis. Additionally, transcription factor analysis revealed that MYB4 and bHLH74 were positively correlated with the contents of theacrine and EGCG3"Me. This study provides valuable materials for further exploring theacrine and EGCG3"Me in tea plants, and establishes a theoretical basis for their biosynthesis.

摘要

茶氨酸和表没食子儿茶素-3-O-(3-O-甲基)没食子酸酯（EGCG3″Me）是茶叶（茶树）中值得注意的次生代谢产物，因其独特风味和显著的健康功效而闻名。茶氨酸对神经刺激有温和作用，而EGCG3″Me表现出更好的稳定性、更高的口服生物利用度和更强的生物活性。然而，天然富含茶氨酸和EGCG3″Me的茶树品种很少见。本研究揭示了一种独特的茶树品种‘安溪苦茶’，其茶氨酸和EGCG3″Me含量都很高。通过整合转录组-蛋白质组-代谢组分析，确定SAMS3、APRT1、IMPDH和TCS1为茶氨酸合成的关键酶；而CHI1、CHI2、FLS2和LAR1是EGCG3″Me合成的关键酶。此外，转录因子分析表明，MYB4和bHLH74与茶氨酸和EGCG3″Me的含量呈正相关。本研究为进一步探索茶树中的茶氨酸和EGCG3″Me提供了有价值的材料，并为它们的生物合成奠定了理论基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/f5c492990644/12870_2025_6691_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/143992b7e785/12870_2025_6691_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/5e9e19000069/12870_2025_6691_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/a30209e0c049/12870_2025_6691_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/fd3464f95cc7/12870_2025_6691_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/e45db1dec7ed/12870_2025_6691_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/f5c492990644/12870_2025_6691_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/143992b7e785/12870_2025_6691_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/5e9e19000069/12870_2025_6691_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/a30209e0c049/12870_2025_6691_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/fd3464f95cc7/12870_2025_6691_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/e45db1dec7ed/12870_2025_6691_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e45e/12087082/f5c492990644/12870_2025_6691_Fig6_HTML.jpg

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本文引用的文献

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