• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

逃脱脂质体自噬体（LAPosome）并以自噬依赖性方式调节巨噬细胞反应。

escapes LAPosome and modulates macrophage response in a xenophagy-dependent manner.

作者信息

Reyne Emilie, Arrindell Jeffrey, Bestion Eloïne, Mezouar Soraya, Desnues Benoit

机构信息

Aix-Marseille Univ, MEPHI, Marseille, France.

IHU-Méditerranée Infection, Marseille, France.

出版信息

Autophagy Rep. 2025 Mar 11;4(1):2475527. doi: 10.1080/27694127.2025.2475527. eCollection 2025.

DOI:10.1080/27694127.2025.2475527
PMID:40395986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11921966/
Abstract

, the agent of Whipple's disease, is an intracellular pathogen that replicates in macrophages. The phagocytic and cellular processes leading to the formation of replicative vacuole remain poorly understood. Macrophage microbicidal activity is largely related to macro/autophagy which is also essential for cell homeostasis. Here, we show that uptake by macrophages involved LC3-associated phagocytosis (LAP). Bacteria then escaped into the cytosol from where they were recaptured by xenophagy. We also demonstrate that blocked the autophagic flux to build its replicative compartment. Inhibition of LAP resulted in the decrease of interleukin (IL)-10 secretion and the restoration of the autophagy flux, suggesting that modulation of autophagy during infection alters immune response and promote persistence. Our results provide new insight in the intracellular fate of the bacteria during macrophage infection and suggest the possible involvement of previously unknown virulence factors in infection.

摘要

惠普尔病的病原体是一种在巨噬细胞内复制的细胞内病原体。导致复制性液泡形成的吞噬和细胞过程仍知之甚少。巨噬细胞的杀菌活性在很大程度上与大自噬/自噬有关，而这对细胞稳态也至关重要。在这里，我们表明巨噬细胞对该病原体的摄取涉及LC3相关吞噬作用（LAP）。然后细菌逃入细胞质，在那里它们被异噬作用重新捕获。我们还证明该病原体阻断自噬通量以构建其复制区室。抑制LAP导致白细胞介素（IL）-10分泌减少和自噬通量恢复，这表明感染期间自噬的调节会改变免疫反应并促进病原体持续存在。我们的结果为巨噬细胞感染期间细菌的细胞内命运提供了新的见解，并表明以前未知的毒力因子可能参与该病原体的感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/9a5e149d4da7/KAUO_A_2475527_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/0ca8f1020765/KAUO_A_2475527_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/adc1fd9dc060/KAUO_A_2475527_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/783d692f3bf6/KAUO_A_2475527_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/35080831f72a/KAUO_A_2475527_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/7b148f9af50c/KAUO_A_2475527_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/9a5e149d4da7/KAUO_A_2475527_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/0ca8f1020765/KAUO_A_2475527_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/adc1fd9dc060/KAUO_A_2475527_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/783d692f3bf6/KAUO_A_2475527_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/35080831f72a/KAUO_A_2475527_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/7b148f9af50c/KAUO_A_2475527_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87d3/11921966/9a5e149d4da7/KAUO_A_2475527_F0006_OC.jpg

相似文献

1
escapes LAPosome and modulates macrophage response in a xenophagy-dependent manner.逃脱脂质体自噬体（LAPosome）并以自噬依赖性方式调节巨噬细胞反应。
Autophagy Rep. 2025 Mar 11;4(1):2475527. doi: 10.1080/27694127.2025.2475527. eCollection 2025.
2
Human galectin-1 and galectin-3 promote infection.人半乳糖凝集素-1 和半乳糖凝集素-3 促进 感染。
Gut Microbes. 2021 Jan-Dec;13(1):1-15. doi: 10.1080/19490976.2021.1884515.
3
Impaired immune functions of monocytes and macrophages in Whipple's disease.Whipple 病中单核细胞和巨噬细胞免疫功能受损。
Gastroenterology. 2010 Jan;138(1):210-20. doi: 10.1053/j.gastro.2009.07.066. Epub 2009 Aug 5.
4
Tropheryma whipplei, the Whipple's disease bacillus, induces macrophage apoptosis through the extrinsic pathway.热带口炎性腹泻棒状杆菌（Tropheryma whipplei），又称惠尔氏病杆菌，通过外在途径诱导巨噬细胞凋亡。
Cell Death Dis. 2010 Apr 8;1(4):e34. doi: 10.1038/cddis.2010.11.
5
Impaired intracellular pathogen clearance and inflammatory joint disease: Is Whipple's disease a guiding light?细胞内病原体清除障碍与炎性关节病：肠源性脂膜炎是否能带来启示？
Joint Bone Spine. 2018 Oct;85(5):531-536. doi: 10.1016/j.jbspin.2017.09.007. Epub 2017 Sep 28.
6
Whipple's disease and Tropheryma whipplei infections: from bench to bedside.惠普尔病与惠普尔养障体感染：从基础到临床。
Lancet Infect Dis. 2022 Oct;22(10):e280-e291. doi: 10.1016/S1473-3099(22)00128-1. Epub 2022 Apr 12.
7
Type I interferon induction is detrimental during infection with the Whipple's disease bacterium, Tropheryma whipplei.Ⅰ型干扰素的诱导在感染 Whipple 病细菌，即屈螺酮体（Tropheryma whipplei）期间是有害的。
PLoS Pathog. 2010 Jan 15;6(1):e1000722. doi: 10.1371/journal.ppat.1000722.
8
Tropheryma whipplei, the agent of Whipple's disease, affects the early to late phagosome transition and survives in a Rab5- and Rab7-positive compartment.惠普尔病的病原体惠普尔嗜组织菌，影响早期到晚期吞噬体的转变，并在Rab5和Rab7阳性区室中存活。
PLoS One. 2014 Feb 24;9(2):e89367. doi: 10.1371/journal.pone.0089367. eCollection 2014.
9
IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation.白细胞介素-16 通过抑制吞噬体转化和调节巨噬细胞活化来促进 whipplei 螺旋体的复制。
PLoS One. 2010 Oct 21;5(10):e13561. doi: 10.1371/journal.pone.0013561.
10
Reduced peripheral and mucosal Tropheryma whipplei-specific Th1 response in patients with Whipple's disease.惠普尔病患者外周和黏膜特异性鞭毛虫Tropheryma whipplei的Th1反应降低。
J Immunol. 2006 Aug 1;177(3):2015-22. doi: 10.4049/jimmunol.177.3.2015.

本文引用的文献

1
The emerging role and therapeutic implications of bacterial and parasitic deubiquitinating enzymes.细菌和寄生虫去泛素化酶的新兴作用和治疗意义。
Front Immunol. 2023 Nov 22;14:1303072. doi: 10.3389/fimmu.2023.1303072. eCollection 2023.
2
Phosphoribosyl-linked serine ubiquitination of USP14 by the SidE family effectors of Legionella excludes p62 from the bacterial phagosome.磷核糖基连接丝氨酸泛素化 USP14 通过军团菌 SidE 家族效应物将 p62 排除在细菌吞噬体之外。
Cell Rep. 2023 Aug 29;42(8):112817. doi: 10.1016/j.celrep.2023.112817. Epub 2023 Jul 19.
3
ESAT-6 a Major Virulence Factor of .
ESAT-6 是 … 的主要毒力因子。
Biomolecules. 2023 Jun 9;13(6):968. doi: 10.3390/biom13060968.
4
Uptake of by Intestinal Epithelia.肠上皮细胞对 的摄取。
Int J Mol Sci. 2023 Mar 24;24(7):6197. doi: 10.3390/ijms24076197.
5
BtpB Manipulates Apoptosis and Autophagic Flux in RAW264.7 Cells.BtpB 调控 RAW264.7 细胞中的细胞凋亡和自噬流。
Int J Mol Sci. 2022 Nov 20;23(22):14439. doi: 10.3390/ijms232214439.
6
Whipple's disease and Tropheryma whipplei infections: from bench to bedside.惠普尔病与惠普尔养障体感染：从基础到临床。
Lancet Infect Dis. 2022 Oct;22(10):e280-e291. doi: 10.1016/S1473-3099(22)00128-1. Epub 2022 Apr 12.
7
Assessment of dopaminergic neuron degeneration in a model of Parkinson's disease.帕金森病模型中多巴胺能神经元变性的评估。
STAR Protoc. 2022 Mar 31;3(2):101264. doi: 10.1016/j.xpro.2022.101264. eCollection 2022 Jun 17.
8
Ubiquitination of the ubiquitin-binding machinery: how early ESCRT components are controlled.泛素化的泛素结合机制：早期 ESCRT 成分是如何被控制的。
Essays Biochem. 2022 Aug 5;66(2):169-177. doi: 10.1042/EBC20210042.
9
Novel and conventional inhibitors of canonical autophagy differently affect LC3-associated phagocytosis.新型和传统的自噬经典途径抑制剂对 LC3 相关的吞噬作用有不同的影响。
FEBS Lett. 2022 Feb;596(4):491-509. doi: 10.1002/1873-3468.14280. Epub 2022 Jan 21.
10
The exploitation of host autophagy and ubiquitin machinery by in shaping immune responses and host defense during infection.在感染过程中， 通过利用宿主自噬和泛素化机制来塑造免疫反应和宿主防御。
Autophagy. 2023 Jan;19(1):3-23. doi: 10.1080/15548627.2021.2021495. Epub 2022 Jan 9.