Kemp R B, Meredith R W, Gamble S H
Food Chem Toxicol. 1985 Feb;23(2):267-70. doi: 10.1016/0278-6915(85)90027-4.
Incubation of LS cells in suspension for 4 hr in the presence of water-soluble commercial products with known Draize rabbit-eye irritation potential has shown that viability, as indicated by fluorescent dyes, is a credible screen for irritation. For product formulations containing immiscible and/or insoluble substances, there are difficulties that have been reduced by extraction of the soluble components of the formulations into the culture medium. A more sensitive and automated, though more expensive, technique based on the estimation of intracellular ATP levels has been developed and the ATP50 values (concentration that reduces intracellular ATP by 50%) for several substances indicate that this may be another suitable endpoint in cytotoxicity screening for animal irritation studies.
将LS细胞在悬浮状态下，于具有已知德雷兹兔眼刺激潜力的水溶性商业产品存在的情况下孵育4小时，结果表明，荧光染料所显示的细胞活力是一种可靠的刺激筛选指标。对于含有不混溶和/或不溶性物质的产品配方，存在一些困难，不过通过将配方中的可溶性成分提取到培养基中，这些困难已有所减少。已经开发出一种基于细胞内ATP水平估计的更灵敏、自动化程度更高但成本也更高的技术，几种物质的ATP50值（使细胞内ATP降低50%的浓度）表明，这可能是动物刺激研究细胞毒性筛选中的另一个合适终点。
