Zhang Guoliang, Wang Zhigang, Jamal Ruxangul, Abdiryim Tursun, Xu Feng, Zhou Yanqiang, Xie Shuyue, Song Kai, Li Jiabei, Ma Lirong, Tan Jing
State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi, 830017, Xinjiang, PR China.
State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, College of Chemical Engineering, Xinjiang University, Urumqi, 830017, Xinjiang, PR China.
Talanta. 2025 Dec 1;295:128364. doi: 10.1016/j.talanta.2025.128364. Epub 2025 May 20.
The molecular imprinting electrochemical sensors (MIECS) currently face issues such as low sensitivity and stability to be improved. This study innovatively combines ZIF-67/TiCTx, treated at high temperature, with molecular imprinting technology to construct a MIECS with excellent stability and high sensitivity. First, size-controllable ZIF-67 was grown in situ on TiCTx, and after high-temperature treatment, Co,N-TiCTx/C was obtained. This was combined with proton-conductive poly(3,4-ethylenedioxythiophene) (PEDOT) to build the MIECS (Co,N-TiCTx/C/PEDOT/MIP/GCE). A series of structural characterization and electrochemical tests revealed that the synergistic effect of the abundant defect structures and the excellent proton conductivity of PEDOT significantly enhanced the electrochemical redox activity. The modified electrode demonstrated outstanding electrochemical performance, including an ultra-high electrochemical active surface area and selective recognition of gatifloxacin (GAT) through the imprinted cavities. Further investigation of the sensor's electrochemical detection performance for GAT showed that the fabricated sensor has high sensitivity, good stability, and excellent selectivity. Experimental results indicated that the sensor has a wide linear response range (0.005-50 μM), a low detection limit (2 nM), and test RSD values are all less than 5 % in honey, milk and lake water, and reliable recoveries were achieved in comparison with standard chromatographic methods. Highlight its potential for practical antibiotic monitoring applications.
目前，分子印迹电化学传感器（MIECS）面临着灵敏度低和稳定性有待提高等问题。本研究创新性地将高温处理后的ZIF-67/TiCTx与分子印迹技术相结合，构建了一种具有优异稳定性和高灵敏度的MIECS。首先，在TiCTx上原位生长尺寸可控的ZIF-67，经过高温处理后，得到Co,N-TiCTx/C。将其与质子导电的聚（3,4-乙撑二氧噻吩）（PEDOT）相结合，构建了MIECS（Co,N-TiCTx/C/PEDOT/MIP/GCE）。一系列结构表征和电化学测试表明，丰富的缺陷结构与PEDOT优异的质子传导性之间的协同效应显著增强了电化学氧化还原活性。修饰电极表现出出色的电化学性能，包括超高的电化学活性表面积以及通过印迹空穴对加替沙星（GAT）的选择性识别。对该传感器检测GAT的电化学性能进一步研究表明，所制备的传感器具有高灵敏度、良好的稳定性和优异的选择性。实验结果表明，该传感器具有宽线性响应范围（0.005 - 50 μM）、低检测限（2 nM），在蜂蜜、牛奶和湖水中的测试相对标准偏差值均小于5%，与标准色谱方法相比具有可靠的回收率。突出了其在实际抗生素监测应用中的潜力。
