Mowafy Rehab E, Megahed Hend M, Khadra Sally H Abou, Bakry Mohamed A, Moustafa Ahmed Hussien, El-Demerdash Azza SalahEldin
Department of Pathology, Agricultural Research Center (ARC), Animal Health Research Institute (AHRI), Zagazig, 44516, Egypt.
Department of Biochemistry, Agricultural Research Center (ARC), Animal Health Research Institute (AHRI), Zagazig, 44516, Egypt.
Sci Rep. 2025 May 22;15(1):17750. doi: 10.1038/s41598-025-01026-x.
This study demonstrates the novel therapeutic potential of Paratapes undulata for mitigating Vibrio alginolyticus infection in red tilapia. In vivo, P. undulata significantly improved growth by approximately 362% in group G3 (Clam-treated Control) and 284% in group G4 (Clam-treated Infected), compared to the infected control group (G2), and reduced mortality by 100% in group G3 and 75% in group G4, compared to the infected control group (G2), and alleviated clinical signs, correlating with enhanced hematological and biochemical profiles, and reduced tissue damage. Mechanistically, P. undulata modulated the immune response by shifting cytokine balance towards anti-inflammation, enhanced antioxidant capacity, and directly inhibited Vibrio alginolyticus virulence. Gas Chromatography-Mass Spectrometry and Fourier-Transform Infrared Spectroscopy analyses revealed the presence of bioactive compounds contributing to these effects. These findings establish P. undulata as a promising, natural, and sustainable biocontrol agent for vibriosis in aquaculture, offering a novel strategy for disease management and reducing reliance on antibiotics. This study suggests that P. undulata can be effectively incorporated into aquaculture feed or water treatments to prevent and manage vibriosis outbreaks.
本研究证明了波纹巴非蛤对减轻红罗非鱼溶藻弧菌感染具有新的治疗潜力。在体内实验中，与感染对照组（G2）相比，G3组（蛤处理对照组）中波纹巴非蛤使生长显著提高了约362%，G4组（蛤处理感染组）中提高了284%；与感染对照组（G2）相比，G3组死亡率降低了100%，G4组降低了75%，并且减轻了临床症状，这与血液学和生化指标的改善以及组织损伤的减轻相关。从机制上讲，波纹巴非蛤通过将细胞因子平衡转向抗炎来调节免疫反应，增强抗氧化能力，并直接抑制溶藻弧菌的毒力。气相色谱-质谱联用和傅里叶变换红外光谱分析揭示了有助于产生这些效应的生物活性化合物的存在。这些发现确立了波纹巴非蛤作为水产养殖中弧菌病一种有前景、天然且可持续的生物防治剂的地位，为疾病管理提供了一种新策略，并减少了对抗生素的依赖。本研究表明，波纹巴非蛤可有效纳入水产养殖饲料或水处理中，以预防和管理弧菌病暴发。
