Network analysis of H. pylori effect on AGS human gastric adenocarcinoma cells gene expression profile.

AIM

To better understand the molecular mechanism of Helicobacter pylori (H. pylori) in adenocarcinoma, the gene expression profile of AGS cells was analyzed by complementary study.

BACKGROUND

Gastric cancer, as one of the most lethal malignancies in the world, is important to be studied in terms of biomarkers. On the other hand, Helicobacter pylori is one of the key risk factors in this type of disease.

METHODS

In this cross-sectional study, we evaluated the seroprevalence of total and IgM anti-HAV antibodies of 254 institutionalized people with intellectual disabilities. Total and IgM anti-HAV antibodies of the blood samples of these people were determined by ELISA method. Protein-protein interaction (PPI) network analysis is a bioinformatic study with validation values for biomarker identification and clarification of molecular mechanisms. Cytoscape V 3.10.2 and its application identified potential central elements of the PPI network and its corresponding roles.

RESULTS

GAPDH and P53 are the most promising candidates in this study. In addition, the microRNA signatures assessment provided more information about these biomarkers and added more value.

CONCLUSION

Consequently, a new outlook for the relationship between gastric cancer and H. pylori was explored based on the new key biomarkers.