Volm M, Drings P, Mattern J, Sonka J, Vogt-Moykopf I, Wayss K
Cancer. 1985 Sep 15;56(6):1396-403. doi: 10.1002/1097-0142(19850915)56:6<1396::aid-cncr2820560630>3.0.co;2-l.
In a cooperative study, 240 surgical specimens of patients with non-small cell lung carcinomas (NSCLC) were investigated by means of flow cytometry, xenotransplantation to athymic mice and, an in vitro short-term test for predicting resistance. Aneuploidy was found in 83% of the tumors, and 20% showed more than one aneuploid DNA stemline. Patients with both aneuploid tumors and tumors with more than one DNA stemline had a significantly shorter survival rate than those with only diploid or only one DNA stemline. Patients whose tumors showed a low G0/G1-cell proportion or a high proliferation pool (S and G2/M-cell proportion) died earlier. A relationship could not be discerned between growth of tumors in nude mice or establishment of cell lines and the prognosis for the patients. Patients with in vitro-resistant tumors died earlier under chemotherapy than those with in vitro-sensitive tumors. Patients treated by radiation survived longer if the tumors were resistant in vitro. Thus, DNA patterns and in vitro short-term tests for predicting resistance represent useful tools for prognostic evaluation of patients with NSCLC.
在一项合作研究中，通过流式细胞术、移植到无胸腺小鼠以及一种预测耐药性的体外短期试验，对240例非小细胞肺癌（NSCLC）患者的手术标本进行了研究。83%的肿瘤中发现了非整倍体，20%显示出不止一个非整倍体DNA干细胞系。既有非整倍体肿瘤又有不止一个DNA干细胞系的肿瘤患者的生存率明显低于仅有二倍体或仅有一个DNA干细胞系的患者。肿瘤显示低G0/G1细胞比例或高增殖池（S和G2/M细胞比例）的患者死亡更早。在裸鼠中肿瘤的生长或细胞系的建立与患者的预后之间未发现关联。体外耐药肿瘤的患者在化疗下比体外敏感肿瘤的患者死亡更早。如果肿瘤在体外耐药，接受放疗的患者存活时间更长。因此，用于预测耐药性的DNA模式和体外短期试验是评估NSCLC患者预后的有用工具。
