Makharita Rabab R, El-Ahmady El-Naggar Noura, Baghdadi Afra Mohammed, Hamouda Ragaa A
Department of Biological Science, College of Science and Arts at Khulis, University of Jeddah, 21959, Jeddah, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
Sci Rep. 2025 Jul 11;15(1):25133. doi: 10.1038/s41598-025-04711-z.
Growing concerns about pollution caused by industrial and agricultural wastes have increased interest in converting waste materials into useful products. Bacterial nanocellulose has garnered global interest due to its environmentally friendly production, excellent mechanical properties, and biocompatibility, making it a promising material for various industries. This study explores the production of bacterial nanocellulose (BNC) by Bacillus strains utilizing fruit waste as a sustainable carbon source. Six potential BNC-producing Bacillus strains were isolated and identified. Among them, Bacillus haynesii showing the highest BNC productivity. A Box-Behnken experimental design was employed to optimize cost-effective technique for BNC production. Key factors like temperature, date waste extract percentage, and initial pH level influencing bacterial cellulose production by Bacillus haynesii were optimized. The highest BNC productivity with a value of 2.6 g/L was obtained under optimized conditions of 29 °C, 15% date waste extract, and pH 6. The glass transition temperature of the bacterial nanocellulose ranged from 21.51 to 42.06 °C, with a low negative charge for colloidal stability. Moreover, crystal violet elimination experiments revealed efficient dye removal (84.7%) with adsorbent concentrations of 2 mg/L and a contact time of 60 min. This study concluded that the Bacillus haynesii 9.1AP strain is a promising candidate for sustainable BNC production. To the best of our knowledge, this study represents the initial documentation of Bacillus haynesii's capability for BNC production, highlighting its potential in environmental and industrial applications, particularly in dye adsorption.
对工农业废弃物造成污染的日益担忧，增加了人们将废料转化为有用产品的兴趣。细菌纳米纤维素因其环保的生产方式、优异的机械性能和生物相容性而受到全球关注，使其成为各行业颇具前景的材料。本研究探索利用水果废料作为可持续碳源，由芽孢杆菌菌株生产细菌纳米纤维素（BNC）。分离并鉴定了六种潜在的产BNC芽孢杆菌菌株。其中，海恩斯芽孢杆菌的BNC生产率最高。采用Box-Behnken实验设计优化BNC生产的经济高效技术。对影响海恩斯芽孢杆菌生产细菌纤维素的温度、枣废料提取物百分比和初始pH值等关键因素进行了优化。在29℃、15%枣废料提取物和pH 6的优化条件下，获得了最高的BNC生产率，为2.6 g/L。细菌纳米纤维素的玻璃化转变温度范围为21.51至42.06℃，具有低负电荷以保持胶体稳定性。此外，结晶紫去除实验表明，在吸附剂浓度为2 mg/L和接触时间为60分钟的情况下，染料去除效率高达84.7%。本研究得出结论，海恩斯芽孢杆菌9.1AP菌株是可持续生产BNC的有前途的候选菌株。据我们所知，本研究首次记录了海恩斯芽孢杆菌生产BNC的能力，突出了其在环境和工业应用中的潜力，特别是在染料吸附方面。
