Eliwa Galal I, El Dengawy El Refaey F, Gawish Mohamed S, Yamany Mona M
Horticulture Department, Faculty of Agriculture, Damietta University, New Damietta, Egypt.
Sci Rep. 2025 May 23;15(1):17905. doi: 10.1038/s41598-025-00848-z.
Rooting is considered a critical stage in the micropropagation of Prunus sp. because it controls plant survival during acclimatization. Auxins and genotype are thought to play a significant role in root formation and induction. Therefore, the objective of the present work was to evaluate the effect of different indole-3-butyric acid (IBA) concentrations on the in vitro rooting and acclimatization of the Okinawa, Nemared, and Garnem peach rootstocks. Microcuttings of 2-3 cm in length were cultivated in MS medium supplemented with IBA (0.0, 0.5, 1.0, 2.0, 3.0, and 4.0 mg L) for the in vitro rooting stage. According to our results, the Garnem genotype exhibited the highest in vitro rooting rate, number of roots per plantlet, and root length. The level of 2.0 mg L of IBA was associated with rooting rates of 100%, 83.33%, and 75% for the Garnem, Okinawa, and Nemared, respectively. The Garnem genotype responded to 4.0 mg L of IBA with a fixed highest root number (12.33), which was the average number of roots per plantlet. Whereas, for the Nemared and Okinawa genotypes, the highest root number per plantlet was 8.00 and 5.00, respectively, in response to 3.0 mg L of IBA. The root lengths of the three genotypes varied significantly depending on the IBA treatment. The Garnem genotype presented the longest root length (5.33 cm), which was followed by the Okinawa genotype (2.49 cm), while the shortest value was presented with the Nemared (1.43 cm). The current study also demonstrated that the three genotypes developed abnormal roots and callus formation when the IBA concentration was increased to 4.00 mg L. Following acclimatization, the Garnem, Okinawa, and Nemared genotypes had respective average survival rates of 93%, 90%, and 75% for plantlets with fully grown shoots and roots.
生根被认为是李属植物微繁殖中的一个关键阶段，因为它控制着驯化过程中植株的存活。生长素和基因型被认为在根系形成和诱导中起着重要作用。因此，本研究的目的是评估不同浓度的吲哚-3-丁酸（IBA）对冲绳、内马雷和加内姆桃砧木离体生根和驯化的影响。在离体生根阶段，将长度为2-3厘米的微插条培养在添加了IBA（0.0、0.5、1.0、2.0、3.0和4.0毫克/升）的MS培养基中。根据我们的结果，加内姆基因型表现出最高的离体生根率、每株幼苗的根数和根长。对于加内姆、冲绳和内马雷，2.0毫克/升的IBA水平分别对应100%、83.33%和75%的生根率。加内姆基因型对4.0毫克/升的IBA反应固定，最高根数为12.33（即每株幼苗的平均根数）。而对于内马雷和冲绳基因型，分别在3.0毫克/升的IBA处理下，每株幼苗的最高根数为8.00和5.00。三种基因型的根长因IBA处理不同而有显著差异。加内姆基因型的根最长（5.33厘米），其次是冲绳基因型（2.49厘米），最短的是内马雷（1.43厘米）。当前研究还表明，当IBA浓度增加到4.00毫克/升时，这三种基因型都会出现异常根和愈伤组织形成。驯化后，对于有完全成熟茎和根的幼苗，加内姆、冲绳和内马雷基因型的平均成活率分别为93%、90%和75%。
