Department of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; Dental Implants Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Biol Macromol. 2023 Aug 30;247:125593. doi: 10.1016/j.ijbiomac.2023.125593. Epub 2023 Jul 3.
Mechanical properties appropriate to native tissues, as an essential component in bone tissue engineering scaffolds, plays a significant role in tissue formation. In the current study, Poly-3 hydroxybutyrate-chitosan (PC) scaffolds reinforced with graphene oxide (GO) were made by the electrospinning method. The addition of GO led to a decrease in fibers diameter, an increase in thermal capacity and an improvement in the surface hydrophilicity of nanocomposite scaffolds. A significant increase in the mechanical properties of PC/GO (PCG) nanocomposite scaffolds was achieved due to the inherent strength of GO as well as its uniform dispersion throughout the polymeric matrix owing to hydrogen bonding and polar interactions. Also, lower biological degradation of the scaffolds (~30% in 100 days) due to the presence of GO provides essential mechanical support for bone regeneration. In addition, the bioactivity results showed that GO reinforcement significantly increases the biomineralization on the surface of the scaffolds. Evaluating cell adhesion and proliferation, as well as ALP activity of MG-63 cells on PC and PCG scaffolds indicated the positive effect of GO on scaffolds' biocompatibility. Overall, the improvement of physicochemical, mechanical, and biological properties of GO-reinforced scaffolds shows the potential of PCG nanocomposite scaffolds for bone tissue engineering.
具有与天然组织相匹配机械性能的支架，作为骨组织工程支架的重要组成部分，在组织形成中起着重要作用。在本研究中，通过静电纺丝法制备了聚-3 羟基丁酸酯-壳聚糖（PC）支架增强氧化石墨烯（GO）。GO 的添加导致纤维直径减小、热容量增加以及纳米复合材料支架的表面亲水性提高。由于 GO 的固有强度以及由于氢键和极性相互作用GO 在聚合物基质中的均匀分散，PC/GO（PCG）纳米复合材料支架的机械性能得到显著提高。此外，由于存在 GO，支架的生物降解率较低（100 天内约为 30%），为骨再生提供了必要的机械支撑。此外，生物活性结果表明，GO 的增强显著增加了支架表面的生物矿化。评估 MG-63 细胞在 PC 和 PCG 支架上的细胞黏附和增殖以及碱性磷酸酶（ALP）活性表明 GO 对支架生物相容性的积极影响。总的来说，GO 增强支架的物理化学、机械和生物学性能的改善表明 PCG 纳米复合材料支架在骨组织工程中的应用潜力。
