Li Peng, Zhang Yalin, Feng Hongjie, Zhou Jinglong, Zhao Lihong, Zhu Heqin, Wei Feng, Feng Zili
State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China.
J Fungi (Basel). 2025 Apr 22;11(5):332. doi: 10.3390/jof11050332.
Verticillium wilt is a devastating soil-borne disease that significantly impacts cotton production, necessitating the development the effective biofumigants for its control. In this study, the inhibitory effect of total volatile organic compounds (VOCs) produced by CEF642 against were evaluated using the one strain many compounds (OSMAC) strategy and analyzed through volatile metabolome. CEF642 was found to produce two primary VOCs, 3-octanol and 2-octenal, (E)-, both of which demonstrated significant antifungal activity. Transcriptome analysis of mycelium grown on various solid media revealed notable differences in the expression of genes associated with arachidonic acid metabolism, lipoxygenase (LOX), and lytic enzymes. These findings provide a foundation for future research aimed at identifying key genes involved in the eight-carbon volatile biosynthetic pathway.
黄萎病是一种极具破坏性的土传病害，严重影响棉花生产，因此需要开发有效的生物熏蒸剂来加以防治。在本研究中，采用一菌多化合物（OSMAC）策略评估了CEF642产生的总挥发性有机化合物（VOCs）对其的抑制作用，并通过挥发性代谢组进行了分析。结果发现CEF642产生两种主要的挥发性有机化合物，即3-辛醇和（E）-2-辛烯醛，二者均表现出显著的抗真菌活性。对在各种固体培养基上生长的菌丝体进行转录组分析，发现与花生四烯酸代谢、脂氧合酶（LOX）和裂解酶相关的基因表达存在显著差异。这些发现为未来旨在鉴定参与八碳挥发性生物合成途径的关键基因的研究奠定了基础。
