Joblin-Mills Aidan, Wu Zhanxuan E, Cooper Garth J S, Sequeira-Bisson Ivana R, Miles-Chan Jennifer L, McGill Anne-Thea, Poppitt Sally D, Fraser Karl
Food Chemistry and Structure, AgResearch Limited, Palmerston North 4442, New Zealand.
High-Value Nutrition National Science Challenge, Auckland 1010, New Zealand.
Metabolites. 2025 Aug 2;15(8):525. doi: 10.3390/metabo15080525.
The causation of type 2 diabetes remains under debate, but evidence supports both abdominal lipid and ectopic lipid overspill into tissues including muscle as key. How these depots differentially alter cardiometabolic profile and change during body weight and fat loss is not known.
Women with obesity scheduled to undergo bariatric surgery were assessed at baseline (BL, = 28) and at 6-month follow-up (6m_FU, = 26) after weight loss. Fasting plasma (Pla), subcutaneous thigh adipose (STA), subcutaneous abdominal adipose, (SAA), and thigh muscle (VLM) samples were collected at BL through surgery and at 6m_FU using needle biopsy. An untargeted liquid chromatography mass spectrometry metabolomics platform was used. Pla and tissue-specific lipid and polar metabolite profiles were modelled as changes from BL and 6m_FU.
There was significant body weight (-24.5 kg) loss at 6m_FU ( < 0.05). BL vs. 6m_FU tissue metabolomics profiles showed the largest difference in lipid profiles in SAA tissue in response to surgery. Conversely, polar metabolites were more susceptible to change in STA and VLM. In Pla samples, both lipid and polar metabolite profiles showed significant differences between timepoints. Jaccard-Tanimoto coefficient t-tests identified a sub-group of gut microbiome and dietary-derived omega-3-fatty-acid-containing lipid species and core energy metabolism and adipose catabolism-associated polar metabolites that are trafficked between sample types in response to bariatric surgery.
In this first report on channelling of lipids and polar metabolites to alternative tissues in bariatric-induced weight loss, adaptive shuttling of small molecules was identified, further promoting adipose processing and highlighting the dynamic and coordinated nature of post-surgical metabolic regulation.
2型糖尿病的病因仍存在争议，但有证据表明腹部脂质和异位脂质过度溢入包括肌肉在内的组织是关键因素。目前尚不清楚这些脂质储存库如何不同地改变心脏代谢特征以及在体重和脂肪减少过程中如何变化。
计划接受减肥手术的肥胖女性在基线时（BL，n = 28）和减肥后6个月随访时（6m_FU，n = 26）接受评估。在基线时通过手术以及在6个月随访时使用针吸活检收集空腹血浆（Pla）、大腿皮下脂肪（STA）、腹部皮下脂肪（SAA）和大腿肌肉（VLM）样本。使用非靶向液相色谱质谱代谢组学平台。将Pla以及组织特异性脂质和极性代谢物谱建模为从基线到6个月随访时的变化。
在6个月随访时体重显著减轻（-24.5 kg）（P < 0.05）。基线与6个月随访时的组织代谢组学谱显示，SAA组织中脂质谱对手术的反应差异最大。相反，极性代谢物在STA和VLM中更容易发生变化。在Pla样本中，脂质和极性代谢物谱在不同时间点之间均显示出显著差异。杰卡德 - 谷本系数t检验确定了一组肠道微生物群和饮食来源的含ω-3脂肪酸的脂质种类以及与核心能量代谢和脂肪分解代谢相关的极性代谢物，它们在减肥手术的作用下在不同样本类型之间进行转运。
在这份关于减肥手术引起体重减轻过程中脂质和极性代谢物向其他组织转运的首次报告中，确定了小分子的适应性穿梭，进一步促进了脂肪处理，并突出了术后代谢调节的动态和协调性。
