Ambata Ambata Henri Tibo, Eyong Moïse Ntah A, Bedine Marie Ampère Boat, Youassi Youassi Yves Olivier, Jiogue Mylaure Benissa, Dikongue Fabiola Jeannette Ndondoni, Tchameni Séverin Nguemezi, Sameza Modeste Lambert
Laboratory of Biochemistry, Department of Biochemistry, Faculty of Science, University of Douala, PO Box 24 147, Douala, Cameroon.
Phytopathology and Agricultural Zoology Research Unit, Department of Agriculture, Faculty of Agronomy and Agronomic Sciences, University of Dschang, P.O. Box 96, Dschang, Cameroon.
Folia Microbiol (Praha). 2025 May 27. doi: 10.1007/s12223-025-01273-5.
This work aimed to evaluate the antagonist effect of Trichoderma viride against Fusarium oxysporum and its ability to promote growth and resistance defence of oil palm. The antagonistic effect of T. viride was tested by paired culture, cellophane plate, and micro-atmosphere methods. In the nursery, 30-day-old oil palm plants were inoculated with T. viride, and 30 days after growing, the roots of plants were infected with the spores of Fusarium oxysporum. Ninety days after infection, plants were harvested, and a series of morphological (height, fresh root and shoot) and biochemical changes (total chlorophyll, phenol compounds, soluble proteins and oxidative enzymes), which are considered to be part of the plant defence response, were evaluated. Results showed that, in dual culture, T. viride significantly reduced mycelial growth of Fusarium oxysporum and released non-volatile and volatile compounds that inhibited the pathogen. In the nursery, there was a significant reduction in disease impact (severity index and incidence) in oil palm inoculated with T. viride and infected with Fusarium oxysporum. A significant increase in plant root and shoot fresh weights, as well as chlorophyll, soluble proteins, and phenol content, was noticed in comparison with non-inoculated control plants. A significant increase in peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase activities was recorded in inoculated and infected plants, compared to control. There was a significant and positive correlation between disease incidence and the activity of these oxidative enzymes. These findings suggest that these compounds play a vital role in plant defence. T. viride could serve as a biological agent to manage vascular wilt in oil palm. However, analysis of its effects on mass production and field application is needed.
本研究旨在评估绿色木霉对尖孢镰刀菌的拮抗作用及其促进油棕生长和抗性防御的能力。通过配对培养、玻璃纸平板和微环境方法测试了绿色木霉的拮抗作用。在苗圃中，对30日龄的油棕植株接种绿色木霉，生长30天后，用尖孢镰刀菌的孢子感染植株根系。感染90天后，收获植株，并评估一系列被认为是植物防御反应一部分的形态学（株高、鲜根和地上部）和生化变化（总叶绿素、酚类化合物、可溶性蛋白和氧化酶）。结果表明，在共培养中，绿色木霉显著降低了尖孢镰刀菌的菌丝生长，并释放出抑制病原体的非挥发性和挥发性化合物。在苗圃中，接种绿色木霉并感染尖孢镰刀菌的油棕植株的病害影响（病情指数和发病率）显著降低。与未接种的对照植株相比，发现植株的根和地上部鲜重以及叶绿素、可溶性蛋白和酚含量显著增加。与对照相比，接种并感染的植株中过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶的活性显著增加。病害发病率与这些氧化酶的活性之间存在显著正相关。这些发现表明这些化合物在植物防御中起着至关重要的作用。绿色木霉可作为一种生物制剂来防治油棕的维管束枯萎病。然而，需要分析其对大规模生产和田间应用的影响。
