Villers D, Derriennic M, Raffi F, Germaud P, Baron D, Nicolas F, Courtieu A L
Chest. 1985 Oct;88(4):527-30. doi: 10.1378/chest.88.4.527.
The reliability of a bronchoscopic protected catheter brush (BPCB) in the diagnosis of lower respiratory tract infection was studied in 17 intubated and ventilated patients, including seven patients free from such infection (group 1) and ten patients with suspected infection (group 2). A first sample was obtained in the lower trachea by aspiration through the fiberoptic bronchoscope and a second in a distal bronchus by the BPCB procedure. In group 1, all BPCB cultures were sterile, although lower tracheal cultures yielded two or more bacterial species, showing that uncontaminated specimens can be obtained by the BPCB procedure. In three patients of group 2, BPCB cultures remained sterile as a nonbacterial pulmonary disease was certified by open lung biopsy. In seven patients from group 2, BPCB cultures yielded all of the organisms isolated simultaneously by reference methods (ie, cultures of blood or pleural fluid, serologic tests, and open lung biopsy). In two of these patients, contamination of the BPCB specimens was ascertained by the reference method bacterial results. In this study the BPCB procedure was able to obtain uncontaminated specimens in intubated and ventilated patients and was mainly accurate in identifying the bacterial etiologic agents of lower respiratory tract infections.
在17例接受插管和机械通气的患者中研究了支气管镜保护性导管刷检（BPCB）在诊断下呼吸道感染方面的可靠性，其中包括7例无此类感染的患者（第1组）和10例疑似感染的患者（第2组）。通过纤维支气管镜吸引在下段气管获取第一份样本，并通过BPCB操作在远端支气管获取第二份样本。在第1组中，尽管下段气管培养物检出两种或更多种细菌，但所有BPCB培养均无菌，这表明通过BPCB操作可获得未受污染的标本。在第2组的3例患者中，由于经开胸肺活检证实为非细菌性肺部疾病，BPCB培养仍无菌。在第2组的7例患者中，BPCB培养检出了所有通过参考方法（即血培养或胸腔积液培养、血清学检测和开胸肺活检）同时分离出的病原体。在其中2例患者中，通过参考方法的细菌检测结果确定了BPCB标本受到污染。在本研究中，BPCB操作能够在接受插管和机械通气的患者中获得未受污染的标本，并且在识别下呼吸道感染的细菌病原体方面基本准确。
