Tița Ovidiu, Onache Petronela Anca, Geana Elisabeta-Irina, Ciucure Corina Teodora, Sumedrea Dorin Ioan, Florea Alina
Department of Agricultural Sciences and Food Engineering, Lucian Blaga University of Sibiu, 550024 Sibiu, Romania.
National Research & Development Institute for Biotechnology in Horticulture Stefanesti-Arges, Sos. Bucuresti-Pitesti, No. 37, 117715 Pitesti, Romania.
Antioxidants (Basel). 2025 May 8;14(5):564. doi: 10.3390/antiox14050564.
The characteristics of the variety from which the wine is made, the geographical area of production, the year of production, and the technology of winemaking are the parameters with the most influence on the total content of polyphenols, the polyphenolic profile, and the antioxidant activity of wine. For this reason, a polyphenolic screen can help establish the authenticity of wines. For this study, 50 samples of white and red wine from different wine areas were collected. For these samples, a qualitative and quantitative analysis was carried out on the polyphenolic profile. The polyphenolic profiles of the studied wines were determined using UHPLC-ESI-MS/MS (mass spectrometry with tandem ionization with high-performance liquid chromatography). Among the non-flavonoid acids, gallic acids, p-coumarnic, and syringic acids in red wines showed higher concentrations in all samples, while resveratrol was present in concentrations from 0.605 to 12.38 mg/L in red wines, and white wines ranged from 0.07 to 0.35 mg/L. For flavonoids, -catechin (0.187 m/L-130.98 mg/L in red wines and 0.04-4.45 mg/L) and (-)-epi-catechin showed the highest concentrations, reaching up to 29.78 mg/L in red wines.
用于酿酒的葡萄品种特性、产地地理区域、生产年份以及酿酒技术是对葡萄酒中多酚总含量、多酚谱和抗氧化活性影响最大的参数。因此,多酚筛选有助于确定葡萄酒的真伪。本研究收集了来自不同葡萄酒产区的50个白葡萄酒和红葡萄酒样本。对这些样本进行了多酚谱的定性和定量分析。使用超高效液相色谱-电喷雾串联质谱法(UHPLC-ESI-MS/MS,即高效液相色谱串联电离质谱法)测定了所研究葡萄酒的多酚谱。在非黄酮酸中,红酒中的没食子酸、对香豆酸和丁香酸在所有样本中浓度较高,而红酒中白藜芦醇的浓度为0.605至12.38毫克/升,白葡萄酒中白藜芦醇的浓度为0.07至0.35毫克/升。对于黄酮类化合物,(+)-儿茶素(红酒中为0.187毫克/升至130.98毫克/升,白葡萄酒中为0.04至4.45毫克/升)和(-)-表儿茶素浓度最高,红酒中可达29.78毫克/升。