Singer Elena, Jung Sun-Hwa, Vivekanand Vivekanand, Lindenberger Christoph
Department of Mechanical Engineering/Environmental Technology, Ostbayerische Technische Hochschule Amberg-Weiden, 92224 Amberg, Germany.
Centre for Energy and Environment, Malaviya National Institute of Technology Jaipur, Jaipur 302017, India.
Bioengineering (Basel). 2025 Apr 26;12(5):460. doi: 10.3390/bioengineering12050460.
The dairy industry generates substantial nutrient-rich wastewater, posing environmental challenges if discharged untreated. This study explores the potential of using the cyanobacterium for nutrient recovery from dairy wastewater, precisely the liquid biogas digestate (BD). The research investigates the feasibility of utilising BD and electrodialysis-concentrated BD (BD concentrate) as alternative media for cultivation, with a focus on biomass productivity, nutrient uptake, and high-value product formation. Batch and continuous cultivation modes were employed. In batch experiments, biomass productivity was in the ratio of 0 and 0.27 g L d, which was 8-100% lower than simulated values for all five tested media compositions. Phosphate fixation was limited with no fixation during batch cultivation and 8-69% during continuous cultivation, likely due to suboptimal N/P ratios, while ammonium removal remained consistently high (>98%). Phycocyanin yield decreased significantly by 92% at high BD concentrate concentrations compared to standard media. Continuous cultivation with 50% BD concentrate improved biomass productivity to 1.02 g L d and pigment yield to 107.9 mg g, suggesting a sufficient supply of nutrients. The findings highlight the potential of BD-based media for nutrient recovery but emphasise the need for optimisation strategies, such as nutrient supplementation and microbial adaptation, to enhance performance.
乳制品行业产生大量富含营养的废水，如果未经处理就排放，会对环境造成挑战。本研究探讨了利用蓝藻从乳制品废水中回收营养物质的潜力，特别是液态沼液（BD）。该研究调查了利用BD和电渗析浓缩BD（BD浓缩液）作为替代培养基进行培养的可行性，重点关注生物量生产力、营养物质吸收和高价值产品的形成。采用了分批培养和连续培养模式。在分批实验中，生物量生产力分别为0和0.27 g L⁻¹ d⁻¹，比所有五种测试培养基组成的模拟值低8 - 100%。磷酸盐固定有限，分批培养期间没有固定作用，连续培养期间为8 - 69%，这可能是由于氮/磷比例不理想，而铵的去除率一直很高（>98%）。与标准培养基相比，在高BD浓缩液浓度下，藻蓝蛋白产量显著下降了92%。使用50%的BD浓缩液进行连续培养可将生物量生产力提高到1.02 g L⁻¹ d⁻¹，并将色素产量提高到107.9 mg g⁻¹，这表明营养物质供应充足。研究结果突出了基于BD的培养基在营养物质回收方面的潜力，但强调需要优化策略，如营养补充和微生物适应，以提高性能。
