Zhang Hongli, Han Zhiping, Zhang Rui, Zhang Yongfang, Wu Juan, Wang Zhichao
School of Agriculture and Life Science, Shanxi Datong University, Datong 037009, China.
Datong Key Laboratory of Organic Dry Farming for Special Crops, Shanxi Datong University, Datong 037009, China.
Genes (Basel). 2025 Apr 28;16(5):509. doi: 10.3390/genes16050509.
Ptinidae, within the infraorder Bostrichiformia, are a cosmopolitan, ecologically diverse but poorly known group. The phylogeny within Bostrichiformia and the monophyly of Ptinidae and its phylogenetic placement in Bostrichiformia remain contentious.
In this research, we determined the entire mitochondrial genome (mitogenome) of , the first representative mitogenome of the subfamily Ptininae, and reconstructed the phylogenetic relationships for Bostrichiformia based on four mitochondrial datasets using maximum likelihood (ML) and Bayesian inference (BI) methods.
The mitogenome of is a circular molecule spanning 17,020 bp and harbors 37 mitochondrial genes and a presumed control region (CR). The mitogenome exhibited a marked preference for the utilization of A and T bases, which was also observed in three kinds of genes and CR. AAT was inferred as the putative candidate initiation codon for cytochrome oxidase subunits 1 (). The control region contains three tandem repeats (TDRs) and one poly-thymine stretch (Poly-T) in both coding strands. The phylogenetic results appeared to support the monophyly of four families, Nosodendridae, Derodontidae, Dermestidae, and Bostrichidae, and the basal position of the latter two families within Bostrichiformia. However, the family Ptinidae was not verified as monophyly because of one species diverging from the main lineage. Three families, Dermestidae, Bostrichidae, and Ptinidae, clustered as the major clade in Bostrichiformia, among which Bostrichidae and Ptinidae grouped together as sister groups.
The present study provides valuable mitochondrial information for Ptinidae and provides novel perspectives on the inner phylogeny within the infraorder Bostrichiformia.
窃蠹科属于长蠹亚目，是一个分布于世界各地、生态多样但鲜为人知的类群。长蠹亚目的系统发育以及窃蠹科的单系性及其在长蠹亚目中的系统发育位置仍存在争议。
在本研究中，我们测定了窃蠹亚科首个代表性线粒体基因组（线粒体基因组），并基于四个线粒体数据集，使用最大似然法（ML）和贝叶斯推断法（BI）重建了长蠹亚目的系统发育关系。
该线粒体基因组是一个环状分子，全长17,020 bp，包含37个线粒体基因和一个假定的控制区（CR）。该线粒体基因组对A和T碱基的使用表现出明显偏好，在三种基因和CR中也观察到这种情况。AAT被推断为细胞色素氧化酶亚基1（）的假定起始密码子。控制区在两条编码链中均包含三个串联重复序列（TDRs）和一个多聚胸腺嘧啶序列（Poly-T）。系统发育结果似乎支持四个科，即窃蠹科、毛薪甲科、皮蠹科和长蠹科的单系性，以及后两个科在长蠹亚目中的基部位置。然而，窃蠹科未被证实为单系，因为有一个物种与主要谱系分化。皮蠹科、长蠹科和窃蠹科这三个科聚为长蠹亚目中的主要分支，其中长蠹科和窃蠹科作为姐妹群聚在一起。
本研究为窃蠹科提供了有价值的线粒体信息，并为长蠹亚目内部的系统发育提供了新的视角。
