Yao Heng, Zheng Yanyan, Sun Yanzhao, Liu Yang
College of Horticulture, China Agricultural University, Beijing 100193, China.
Int J Mol Sci. 2025 May 14;26(10):4675. doi: 10.3390/ijms26104675.
The FAR1-RELATED SEQUENCE (FRS) family consists of transcription factors derived from transposases, playing crucial roles in plants by mediating processes such as light signaling transduction, hormone response and stress resistance. Despite the ecological and economic importance of grapes, systematic research on FRS genes in this species is limited. In this study, we identified 43 genes, distributed across 13 chromosomes in grape. Phylogenetic analysis was conducted on the VvFRS family members along with Arabidopsis, tomato, and strawberry, resulting in their classification into seven distinct subgroups. Our predictions indicated that most VvFRSs were localized within the nucleus, with predominant secondary structures of α-helices and random coils. Additionally, it was observed that genes within the same subgroup exhibited a similar distribution of conserved motifs. The promoter regions of the gene family harbored multiple cis-elements associated with abiotic stress, hormone response, and light signaling pathways. The RT-qPCR results revealed that the expression levels of genes in subgroups I and IV exhibited the highest abundance in leaves. Certain genes demonstrated significant responsiveness to salt stress and jasmonic acid treatment. This study presents the first comprehensive identification of FRS genes in grape, offers a foundation for further functional studies.
FAR1相关序列（FRS）家族由源自转座酶的转录因子组成，通过介导光信号转导、激素反应和抗逆性等过程在植物中发挥关键作用。尽管葡萄具有生态和经济重要性，但对该物种中FRS基因的系统研究有限。在本研究中，我们鉴定了43个基因，它们分布在葡萄的13条染色体上。对葡萄VvFRS家族成员以及拟南芥、番茄和草莓进行了系统发育分析，结果将它们分为七个不同的亚组。我们的预测表明，大多数VvFRS定位于细胞核内，主要二级结构为α-螺旋和无规卷曲。此外，观察到同一亚组内的基因具有相似的保守基序分布。该基因家族的启动子区域含有多个与非生物胁迫、激素反应和光信号通路相关的顺式元件。RT-qPCR结果显示，亚组I和IV中的基因在叶片中的表达水平最高。某些基因对盐胁迫和茉莉酸处理表现出显著响应。本研究首次全面鉴定了葡萄中的FRS基因，为进一步的功能研究提供了基础。
