Wójcik Barbara, Zawadzka Katarzyna, Hotowy Anna, Jóźwiak Maria, Jusińska Klaudia, Wierzbicki Mateusz
Department of Nanobiotechnology, Warsaw University of Life Science, Ciszewskiego 8, 02-786 Warsaw, Poland.
Int J Mol Sci. 2025 May 16;26(10):4761. doi: 10.3390/ijms26104761.
Several scientific studies have reported the opposing effects of silver nanoparticles (AgNPs) on angiogenesis, ranging from proangiogenic to anti-angiogenic. The widespread use of AgNPs in biomedical applications and the variability of their effects depending on concentration and exposure conditions highlight the need for further research into their impact on vascularization and endothelial cell behavior. This study aimed to investigate the potential influence of AgNPs on human umbilical vein endothelial cells (HUVECs) using a model incorporating a thin layer of an extracellular matrix (ECM). To this end, cytotoxicity was assessed, and endogenous nitric oxide and superoxide levels were measured. Additionally, the effects of AgNPs on HUVEC confluence and migration were evaluated. The expression levels of 43 proteins involved in angiogenesis were also analyzed. The results revealed that ECM enriched with AgNPs at a concentration of 0.5 mg/L enhanced cell coverage, promoted migration, and supported monolayer formation without inducing cytotoxicity.
多项科学研究报告了银纳米颗粒（AgNPs）对血管生成的相反作用，从促血管生成到抗血管生成。AgNPs在生物医学应用中的广泛使用以及其作用因浓度和暴露条件而异，凸显了进一步研究其对血管形成和内皮细胞行为影响的必要性。本研究旨在使用包含细胞外基质（ECM）薄层的模型，研究AgNPs对人脐静脉内皮细胞（HUVECs）的潜在影响。为此，评估了细胞毒性，并测量了内源性一氧化氮和超氧化物水平。此外，还评估了AgNPs对HUVEC汇合和迁移的影响。还分析了43种参与血管生成的蛋白质的表达水平。结果显示，浓度为0.5 mg/L的富含AgNPs的ECM可增强细胞覆盖、促进迁移并支持单层形成，且不会诱导细胞毒性。
