Hussain Z, Lannigan R, Stoakes L
Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Oct;258(1):74-9. doi: 10.1016/s0176-6724(84)80011-5.
Several tests, bacitracin, CAMP, pyroglutamic acid-B-naphthylamide test (PYR test), bile aesculin hydrolysis, salt tolerance and pigment production tests were evaluated for their reliability and speed in presumptive identification of streptococci. Bacitracin correctly identified all of the group A streptococci but 5% of other beta haemolytic streptococci were misidentified as group A. The PYR reaction was just as sensitive but more specific for group A streptococci and the results were available within 2-3 h. The PYR reaction also effectively differentiated between enterococcal and non-enterococcal group D streptococci. Both the salt tolerance test and the PYR reaction misidentified bile aesculin positive non-group D streptococci as non-enterococcal group D streptococci. Pigment production by group B streptococci was more reliable than the CAMP test for identifying group B streptococci. The combination of PYR, bile aesculin and pigment production allowed rapid differentiation of several medically important groups of streptococci.
对杆菌肽、CAMP、焦谷氨酸-β-萘酰胺试验（PYR试验）、胆汁七叶苷水解试验、耐盐试验和色素产生试验等多项试验进行了评估，以确定它们在初步鉴定链球菌时的可靠性和速度。杆菌肽能正确鉴定所有A群链球菌，但5%的其他β溶血性链球菌被误鉴定为A群。PYR反应对A群链球菌同样敏感，但特异性更强，结果可在2-3小时内得出。PYR反应还能有效区分肠球菌和非肠球菌D群链球菌。耐盐试验和PYR反应都将胆汁七叶苷阳性的非D群链球菌误鉴定为非肠球菌D群链球菌。B群链球菌产生色素比CAMP试验在鉴定B群链球菌时更可靠。PYR、胆汁七叶苷和色素产生试验的组合能够快速区分几种具有重要医学意义的链球菌群。
