用于A群β溶血性链球菌和D群肠球菌初步鉴定的Strep-A-Chek技术评估。

Evaluation of the Strep-A-Chek technique for presumptive identification of group A beta-hemolytic streptococci and group D enterococci.

作者信息

Daly J A, Rufener M L

机构信息

Primary Children's Medical Center, Salt Lake City, UT 84103.

出版信息

Diagn Microbiol Infect Dis. 1987 Jul;7(3):215-8. doi: 10.1016/0732-8893(87)90008-3.

DOI:10.1016/0732-8893(87)90008-3

PMID:3308307

Abstract

Strep-A-Check (E-Y Laboratories, San Mateo, CA) is a 15-min chromogenic test for a species-specific aminopeptidase that could replace testing bacitracin susceptibility for presumptive identification of group A streptococci as well as 6.5% NaCl agar tolerance for presumptive identification of enterococcal streptococci, with a time savings of 24 hr. Recent clinical streptococcal isolates (n = 341), identified by conventional biochemical and serologic techniques, were used to evaluate the 15-min Strep-A-Chek test. Among the beta-hemolytic streptococci (176 group A, 43 group B, 8 group C, 9 group F, and 9 group G), Strep-A-Chek was 100% accurate. Among the non-beta-hemolytic streptococci, 100% of 52 group D enterococci, 100% of 30 viridans streptococci, and 100% of 14 group D nonenterococci were correctly identified by Strep-A-Chek. Strep-A-Chek is an extremely rapid and reliable test for presumptive identification of group A and enterococcal streptococci.

摘要

链球菌A检测试剂盒（E-Y实验室，加利福尼亚州圣马特奥）是一种用于检测特定物种氨肽酶的15分钟显色试验，它可以替代检测杆菌肽敏感性以初步鉴定A群链球菌，以及替代6.5%氯化钠琼脂耐受性试验以初步鉴定肠球菌性链球菌，可节省24小时时间。采用传统生化和血清学技术鉴定的近期临床链球菌分离株（n = 341）用于评估15分钟的链球菌A检测试验。在β溶血性链球菌（176株A群、43株B群、8株C群、9株F群和9株G群）中，链球菌A检测试验的准确率为100%。在非β溶血性链球菌中，链球菌A检测试验正确鉴定出了52株D群肠球菌中的100%、30株草绿色链球菌中的100%以及14株D群非肠球菌中的100%。链球菌A检测试验是一种用于初步鉴定A群和肠球菌性链球菌的极其快速且可靠的试验。