Sventzouri Eirini, Pispas Konstantinos, Kournoutou Georgia G, Geroulia Maria, Giakoumatou Eleni, Ali Sameh Samir, Kornaros Michael
Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 26504 Patras, Greece.
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
Microorganisms. 2025 May 19;13(5):1157. doi: 10.3390/microorganisms13051157.
Large-scale cultivation of cyanobacteria is often limited by the high cost of synthetic culture medium and the environmental impact of nutrient consumption. Cheese whey, a major agro-industrial waste product, is rich in organic and inorganic nutrients, making it a promising low-cost alternative for microbial growth while addressing waste bioremediation. This study investigates the growth performance and the biochemical composition of four different cyanobacterial species ( sp., sp., and ), cultivated in cheese whey (CW). Pretreated CW was used at 20% and 100% / concentrations. All species grew satisfactorily in both concentrations, reaching biomass above 4 g L (in 100% / CW) and 2 g L (in 20% / CW). The highest μ value (0.28 ± 0.02 d) was presented by sp. grown in 20% CW. Waste bioremediation of both 20 and 100% / CW demonstrated effective nutrient removal, with COD removal exceeding 50% for most species, while total nitrogen (TN) and total phosphorus (TP) removals reached up to 33% and 32%, respectively. Biochemical composition analysis revealed high carbohydrate and protein content, while lipid content remained below 15% in all cases. Interestingly, accumulated 11% / polyhydroxyalkanoates (PHAs) during the last day of cultivation in 20% / CW. These findings highlight the potential of as a valuable candidate for integration into bioprocesses aimed at sustainable bioplastic production. Its ability to synthesize PHAs from agro-industrial waste not only enhances the economic viability of the process but also aligns with circular economy principles. This study is a primary step towards establishing a biorefinery concept for the cultivation of cyanobacterial species in cheese whey-based wastewater streams.
蓝藻的大规模培养常常受到合成培养基高成本以及营养物质消耗对环境影响的限制。奶酪乳清是一种主要的农业工业废弃物，富含有机和无机营养物质，这使其成为微生物生长有前景的低成本替代品，同时还能解决废弃物生物修复问题。本研究调查了在奶酪乳清（CW）中培养的四种不同蓝藻物种（ 种、 种、 种和 种）的生长性能和生化组成。预处理后的CW以20%和100% / 的浓度使用。所有物种在这两种浓度下均生长良好，在100% / CW中生物量达到4 g L以上，在20% / CW中达到2 g L以上。在20% CW中生长的 种呈现出最高的μ值（0.28 ± 0.02 d）。对20%和100% / CW的废弃物生物修复均显示出有效的营养物质去除效果，大多数物种的化学需氧量（COD）去除率超过50%，而总氮（TN）和总磷（TP）去除率分别高达33%和32%。生化组成分析表明碳水化合物和蛋白质含量较高，而在所有情况下脂质含量均低于15%。有趣的是，在20% / CW培养的最后一天， 积累了11% / 的聚羟基脂肪酸酯（PHA）。这些发现突出了 作为整合到旨在可持续生物塑料生产的生物过程中的有价值候选者的潜力。其从农业工业废弃物中合成PHA的能力不仅提高了该过程的经济可行性，还符合循环经济原则。本研究是朝着建立基于奶酪乳清的废水流中蓝藻物种培养的生物炼制概念迈出的第一步。
