Zhang Yuyin, Jiang Zixiao, Dou Hua, Fan Chenlu, Wei Jianhe, Chen Xuyu
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Haikou, Hainan, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Front Plant Sci. 2025 May 13;16:1546050. doi: 10.3389/fpls.2025.1546050. eCollection 2025.
Agarwood, a prized aromatic resin from , is formed as a defensive response to injury or fungal infection. However, the factors influencing its chemical composition remain poorly understood. This study aimed to explore the relationship between endophytic fungal communities and volatile oil content in ordinary-type and Qinan-type .
Using high-throughput sequencing, we analyzed the fungal composition in both the healthy wood and agarwood layers of different germplasms. Additionally, gas chromatography-mass spectrometry (GC-MS) was employed to quantify the volatile oil content.
The results revealed that the fungal community composition in the agarwood layer differed between the two types of , with Fusarium, Hermatomyces, and Rhinocladiella linked to sesquiterpene production (r>0.8, p<0.01), while Microidium, Cladosporium, and Cephalotrichum were associated with chromone levels (r>0.8, p<0.01). Furthermore, the volatile oil content in Qinan-type agarwood was significantly higher than that in ordinary-type agarwood, with distinct chemical profiles observed in each germplasm.
These findings provide critical insights into the role of endophytic fungi in shaping agarwood's chemical composition and have practical implications for enhancing agarwood production in the industry. Consequently, this research has significant implications for the agarwood industry, as it enhances our understanding of how fungi influence resin quality and paves the way for improving the efficiency of agarwood induction, ultimately leading to higher-quality and more sustainable production.
沉香是一种珍贵的芳香树脂，由沉香树对损伤或真菌感染的防御反应形成。然而，影响其化学成分的因素仍知之甚少。本研究旨在探讨普通型和奇楠型沉香内生真菌群落与挥发油含量之间的关系。
我们使用高通量测序分析了不同沉香种质健康木材和沉香层中的真菌组成。此外，采用气相色谱 - 质谱联用（GC-MS）对挥发油含量进行定量分析。
结果表明，两种类型沉香的沉香层中真菌群落组成不同，镰刀菌、腐皮镰刀菌和枝顶孢属与倍半萜的产生有关（r>0.8，p<0.01），而小孢发菌、枝孢属和头孢霉属与色酮水平有关（r>0.8，p<0.01）。此外，奇楠型沉香的挥发油含量显著高于普通型沉香，且每种种质的化学特征各不相同。
这些发现为内生真菌在塑造沉香化学成分中的作用提供了关键见解，并对提高沉香产业的产量具有实际意义。因此，本研究对沉香产业具有重要意义，因为它增强了我们对真菌如何影响树脂质量的理解，并为提高沉香诱导效率铺平了道路，最终实现更高质量和更可持续的生产。
