Cho Sangyeon, Moon Wonjoon, Martino Nicola, Yun Seok Hyun
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Cambridge, Massachusetts, 02139, USA.
Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.
Adv Mater. 2025 May 28:e2418710. doi: 10.1002/adma.202418710.
Laser particles (LPs) emitting narrowband spectra across wide spectral ranges are highly promising for high-multiplex optical barcoding of biological cells. Here, LPs based on indium phosphide (InP) nanodisks are presented, operating in the near-infrared wavelength range of 740-970 nm. Utilizing low-order whispering gallery resonance modes in size-tuned nanodisks, an ultrawide color palette with 25% spectral utilization and nanometer-scale linewidth is achieved. A simple theoretical model accurately predicts spectral ranges based on particle size. The minimum laser size is 430 nm in air and 560 nm within cells, operating at mode orders of 4 or 5. The high brightness and narrow linewidths of polymer-silica-protected InP LPs, combined with a silicon-detector spectrometer, enable spectral detection of laser peaks with high signal-to-background ratios in highly-scattering media, including 1-cm-thick chicken breast tissue and blood vessels in live mice.
能在宽光谱范围内发射窄带光谱的激光粒子（LPs）在生物细胞的高复用光学条形码技术方面极具潜力。本文展示了基于磷化铟（InP）纳米盘的LPs，其工作在740 - 970纳米的近红外波长范围内。通过利用尺寸调谐纳米盘中的低阶回音壁共振模式，实现了具有25%光谱利用率和纳米级线宽的超宽颜色调色板。一个简单的理论模型能根据粒子大小准确预测光谱范围。在空气中，最小激光粒子尺寸为430纳米，在细胞内为560纳米，工作在4或5的模式阶数下。聚合物 - 二氧化硅保护的InP LPs的高亮度和窄线宽，结合硅探测器光谱仪，能够在高散射介质中以高信噪比光谱检测激光峰，包括1厘米厚的鸡胸组织和活体小鼠的血管。
