血清中乙肝e抗原/抗体与乙肝病毒脱氧核糖核酸的不一致性。对1063份标本的分析。

Discordance of hepatitis B e antigen/antibody and hepatitis B virus deoxyribonucleic acid in serum. Analysis of 1063 specimens.

作者信息

Matsuyama Y, Omata M, Yokosuka O, Imazeki F, Ito Y, Okuda K

出版信息

Gastroenterology. 1985 Nov;89(5):1104-8. doi: 10.1016/0016-5085(85)90216-1.

Abstract

Hepatitis B virus DNA was determined in 1063 serum samples from 252 patients with hepatitis B surface antigen by the spot hybridization technique. The results were correlated with hepatitis B e antigen and antibody. Hepatitis B virus DNA was detected in 87% of hepatitis B e antigen-positive patients, in 18% of hepatitis B e antibody-positive patients, and in 18% of those negative for both. Discordance of antigen/antibody and hepatitis B virus DNA, i.e., the presence of the DNA in antibody-positive sera or the absence of the DNA in antigen-positive sera, was observed in 209 of 997 (21%) samples. Of 121 patients with histologic diagnosis, this discordance was observed in none of 20 patients with nonspecific changes, in 13% of 39 with chronic persistent hepatitis, in 21% of 38 with chronic active hepatitis, and in 38% of 24 with cirrhosis. Thus, hepatitis B e antigen/antibody testing alone failed to predict the presence or absence of circulating hepatitis B virion in a significant proportion of patients with advanced chronic liver disease.

摘要

采用斑点杂交技术对252例乙型肝炎表面抗原阳性患者的1063份血清样本进行了乙肝病毒DNA检测。结果与乙肝e抗原和抗体进行了相关性分析。乙肝e抗原阳性患者中87%检测到乙肝病毒DNA，乙肝e抗体阳性患者中18%检测到，两者均阴性的患者中18%检测到。在997份样本中的209份（21%）中观察到抗原/抗体与乙肝病毒DNA不一致，即抗体阳性血清中存在DNA或抗原阳性血清中不存在DNA。在121例经组织学诊断的患者中，20例非特异性改变患者均未观察到这种不一致，39例慢性持续性肝炎患者中有13%，38例慢性活动性肝炎患者中有21%，24例肝硬化患者中有38%观察到这种不一致。因此，仅检测乙肝e抗原/抗体无法在相当比例的晚期慢性肝病患者中预测循环乙肝病毒颗粒的存在与否。