乙肝表面抗原无症状携带者血清中e抗原与 Dane 颗粒 DNA 的关联
Association of e antigen with Dane particle DNA in sera from asymptomatic carriers of hepatitis B surface antigen.
作者信息
Werner B G, O'Connell A P, Summers J
出版信息
Proc Natl Acad Sci U S A. 1977 May;74(5):2149-51. doi: 10.1073/pnas.74.5.2149.
Sera containing hepatitis B surface antigen from 30 asymptomatic blood donors were assayed for e antigen (HBeAg) and antibody to e antigen (anti-HBe) by rheophoresis,. Fourteen samples (47%) had detectable HBeAg, ten (33%) had anti-HBe, and six (20%) had neither. DNA was extracted from 26 of these sera and assayed for its ability to anneal to a [32P]-DNA probe that is a copy of Dane particle DNA. All 10 HBeAg-positive samples tested contained DNA that formed specific hybrids with the DNA probe, as did one of 10 anti HBe-positive samples. Hybridization was not detected in nine sera containing anti-HBe and six sera without HBeAg or anti-HBe. Because the Dane particle is thought to be the hepatitis B virus, this association between HBeAg positivity and Dane particle DNA strongly supports the hypothesis that e antigen is a marker of the presence of the virus and, consequently, potential infectivity.
通过电流泳法对30名无症状献血者的含乙肝表面抗原血清进行e抗原（HBeAg）和e抗原抗体（抗-HBe）检测。14份样本（47%）可检测到HBeAg，10份（33%）有抗-HBe，6份（20%）两者均未检测到。从其中26份血清中提取DNA，并检测其与作为Dane颗粒DNA拷贝的[32P]-DNA探针退火的能力。所有检测的10份HBeAg阳性样本所含的DNA均与DNA探针形成特异性杂交体，10份抗-HBe阳性样本中的1份也是如此。在9份含抗-HBe的血清以及6份既无HBeAg也无抗-HBe的血清中未检测到杂交。由于Dane颗粒被认为是乙肝病毒，HBeAg阳性与Dane颗粒DNA之间的这种关联有力地支持了以下假说：e抗原是病毒存在的标志物，因此也是潜在传染性的标志物。
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