Na Qin, Amu Gu-Leng, Mei Li, Gui Zhi, Song Lin
College of Mongolian Medicine, Inner Mongolia Medical University, Hohhot, China.
Department of Pharmacy, Inner Mongolia International Mongolian Hospital, Hohhot, China.
J Vet Med Sci. 2025 Jul 11;87(7):854-861. doi: 10.1292/jvms.24-0414. Epub 2025 May 28.
Due to the high level of heavy metals and harmful elements, United States and Europe are precautious in importing herbal medicines, which has seriously hindered the internationalization process of traditional Chinese and Mongolian medicines. Ga-Mu-Zhu-Er (GMZE) contains a wide range of chemical compounds and inorganic elements. This paper describes a validation process for the simultaneous analysis of 30 elements in Traditional Mongolian Medicine GMZE samples with closed-vessel microwave digestion technique followed by inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The samples digested with concentrated nitric acid (65%, v/v) revealed recoveries ranging from 91.9 to 107.7%. Several other parameters, including linearity, specificity, the limit of detection (LOD) and quantification (LOQ), precision of measurement, repeatability, intermediate precision and accuracy, were validated. The LOD were found to be in the range of 0.00015-0.21 µg/L, and the LOQ were between 0.00046 to 0.64 µg/L. Three GMZE samples were analyzed. Among the analyzed elements, Ca, As, and Hg were the most abundant, whereas, Be and Ag were present in low concentrations in all the samples. The results obtained in our study demonstrated that the developed ICP-MS method is simple, fast, and reliable, which could be used for the routine quantitation of these 30 essential and non-essential trace elements in GMZE as well as other Traditional Chinese Medicines and dietary supplements, with acceptable analytical performance.
由于重金属和有害元素含量较高，美国和欧洲在进口草药时持谨慎态度，这严重阻碍了中药和蒙药的国际化进程。嘎木朱尔（GMZE）含有多种化合物和无机元素。本文描述了一种采用密闭容器微波消解技术，随后进行电感耦合等离子体质谱（ICP-MS）分析，同时测定传统蒙药GMZE样品中30种元素的验证过程。用浓硝酸（65%，v/v）消解的样品回收率在91.9%至107.7%之间。还对包括线性、特异性、检测限（LOD）和定量限（LOQ）、测量精密度、重复性、中间精密度和准确度等其他几个参数进行了验证。检测限在0.00015 - 0.21 μg/L范围内，定量限在0.00046至0.64 μg/L之间。对三个GMZE样品进行了分析。在所分析的元素中，钙、砷和汞含量最高，而铍和银在所有样品中的浓度都很低。我们研究中获得的结果表明，所开发的ICP-MS方法简单、快速且可靠，可用于GMZE以及其他中药和膳食补充剂中这30种必需和非必需微量元素的常规定量分析，具有可接受的分析性能。
