Ndumbe P M, Levinsky R J
Immunology. 1985 Sep;56(1):189-94.
We have described the optimal conditions for the culture of human fibroblast cells from whole bone marrow aspirates and have shown that these fibroblasts differ in surface markers from those obtained from the skin and embryo lung tissue. All three fibroblast types express HLA class 1 antigens, together with an antigen found on T cells, granulocytes, white matter of brain and breast epithelial cells, but none of them expressed HLA class 2 antigens. In addition, skin fibroblasts expressed an antigen found on common acute lymphoblastic leukaemia cells, while bone marrow fibroblasts expressed an antigen found on human natural killer cells. Hence, all three types of fibroblasts can be separated on the basis of their surface markers, thereby suggesting a difference in maturation pathways.
我们已经描述了从全骨髓抽吸物中培养人成纤维细胞的最佳条件，并表明这些成纤维细胞在表面标志物上与从皮肤和胚胎肺组织获得的成纤维细胞不同。所有三种类型的成纤维细胞均表达HLA-Ⅰ类抗原，以及一种在T细胞、粒细胞、脑白质和乳腺上皮细胞上发现的抗原，但它们均不表达HLA-Ⅱ类抗原。此外，皮肤成纤维细胞表达一种在常见急性淋巴细胞白血病细胞上发现的抗原，而骨髓成纤维细胞表达一种在人类自然杀伤细胞上发现的抗原。因此，所有三种类型的成纤维细胞都可以根据其表面标志物进行分离，从而表明成熟途径存在差异。
