Liu Han, Zhao Jing, Zhang Wenju, Nie Cunxi
College of Animal Science and Technology, Shihezi University, Shihezi, China.
Front Microbiol. 2023 Jan 12;13:1041885. doi: 10.3389/fmicb.2022.1041885. eCollection 2022.
Butyrate is thought to enhance intestinal mucosal homeostasis, but the detailed mechanism remains unclear. Therefore, further investigation on the mechanism of butyrate regulation of intestinal mucosal homeostasis was performed.
This study used weaned piglets with similar intestinal metabolic function to humans as a research model. The dietary supplemented 0.2% sodium butyrate group (0.2% S) and negative control group (CON) were established to detect the effects of butyrate on growth performance, intestinal tissue morphology, mucosal barrier function, and intestinal microbial community structure in weaned piglets.
There was an increase in average daily gain (ADG) during three different experimental periods and a reduction in average daily feed intake (ADFI) and feed-to-gain ratio (F:G) during days 1-35 and days 15-35 in 0.2% S compared with CON ( > 0.05). Furthermore, villus height in the ileum and duodenum was increased, and crypt depths in the colon and jejunum were reduced in both groups ( < 0.05). Moreover, the ratio of villus height and crypt depth (V/C) in 0.2% S both in the ileum and jejunum was significantly increased ( < 0.05) compared with CON. The relative mRNA expression of , , , and was upregulated in the ileum of 0.2% S compared with CON ( < 0.05). The digesta samples of 0.2% S, both in the ileum ( < 0.05) and colon, contained greater intestinal bacterial abundance and diversity of probiotics, including , , , and , which promoted amino acid metabolism and energy production and conversion in the colon and the synthesis of carbon-containing biomolecules in the ileum.
In summary, dietary supplementation with 0.2% sodium butyrate was shown to have a tendency to improve the growth performance of weaned piglets and enhance intestinal mucosal barrier function altering the gut microbiota.
丁酸被认为可增强肠道黏膜稳态，但其具体机制仍不清楚。因此，对丁酸调节肠道黏膜稳态的机制进行了进一步研究。
本研究以肠道代谢功能与人类相似的断奶仔猪作为研究模型。设立添加0.2%丁酸钠的日粮组（0.2% S）和阴性对照组（CON），以检测丁酸对断奶仔猪生长性能、肠道组织形态、黏膜屏障功能和肠道微生物群落结构的影响。
与CON组相比，0.2% S组在三个不同实验期的平均日增重（ADG）有所增加，在第1 - 35天和第15 - 35天的平均日采食量（ADFI）和料重比（F:G）有所降低（P>0.05）。此外，两组回肠和十二指肠的绒毛高度均增加，结肠和空肠的隐窝深度均降低（P<0.05）。而且，与CON组相比，0.2% S组回肠和空肠的绒毛高度与隐窝深度之比（V/C）显著增加（P<0.05）。与CON组相比，0.2% S组回肠中、、、的相对mRNA表达上调（P<0.05）。0.2% S组回肠（P<0.05）和结肠的食糜样本中，肠道细菌丰度更高，益生菌种类更多，包括、、、，这些益生菌促进了结肠中的氨基酸代谢以及能量产生和转化，以及回肠中含碳生物分子的合成。
综上所述，日粮中添加0.2%丁酸钠显示出有改善断奶仔猪生长性能和增强肠道黏膜屏障功能的趋势，同时改变了肠道微生物群。
