Deepe G S, Taylor C L, Bullock W E
Infect Immun. 1985 Oct;50(1):183-9. doi: 10.1128/iai.50.1.183-189.1985.
A reproducible model of disseminated blastomycosis was established in C57BL/6 mice by intravenous injection of 10(6) yeast-phase Blastomyces dermatiditis organisms. The infection progressed over 5 weeks to involve lungs, brains, superficial fascia, livers, and spleens of mice. By week 5, there was a greater number of organisms in lungs and brains than in livers and spleens. The tissue response in lungs, brains, and livers progressed from acute neutrophilic invasion before week 1 to pyogranuloma formation by week 5. Lymph nodes and spleens were remarkably spared. By week 5, infected mice became anergic to intradermal challenge with both specific Blastomyces antigen and a nonspecific antigen (sheep erythrocytes). At this time, the response to concanavalin A or phytohemagglutinin by splenocytes was markedly less than that of normal controls. Likewise, the plaque-forming cell response to sheep erythrocytes by splenocytes from infected mice was diminished. In coculture studies, splenocytes from 5-week-infected mice reduced the plaque-forming cell response by normal splenocytes. The development of this murine model should prove useful for elucidating the perturbations of immunoregulation associated with disseminated blastomycosis.
通过静脉注射10(6)个酵母相皮炎芽生菌，在C57BL/6小鼠中建立了可重复的播散性芽生菌病模型。感染在5周内进展，累及小鼠的肺、脑、浅筋膜、肝和脾。到第5周时，肺和脑中的菌量比肝和脾中的更多。肺、脑和肝中的组织反应从第1周前的急性嗜中性粒细胞浸润发展到第5周时形成脓性肉芽肿。淋巴结和脾明显未受影响。到第5周时，感染小鼠对皮内注射特异性芽生菌抗原和非特异性抗原（绵羊红细胞）均无反应。此时，脾细胞对刀豆球蛋白A或植物血凝素的反应明显低于正常对照。同样，感染小鼠脾细胞对绵羊红细胞的空斑形成细胞反应也减弱。在共培养研究中，感染5周小鼠的脾细胞降低了正常脾细胞的空斑形成细胞反应。该小鼠模型的建立对于阐明与播散性芽生菌病相关的免疫调节紊乱应是有用的。
