通过酶联免疫吸附测定法在气单胞菌属培养物中检测霍乱毒素相关因子
Demonstration of cholera toxin-related factor in cultures of Aeromonas species by enzyme-linked immunosorbent assay.
作者信息
Honda T, Sato M, Nishimura T, Higashitsutsumi M, Fukai K, Miwatani T
出版信息
Infect Immun. 1985 Oct;50(1):322-3. doi: 10.1128/iai.50.1.322-323.1985.
Abstract
The production of toxins by Aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. A factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of Aeromonas hydrophila and 4 of 15 strains of Aeromonas sobria. Analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mouse test.
摘要
通过乳鼠试验、溶血素试验以及使用抗霍乱肠毒素的酶联免疫吸附测定法检测了气单胞菌属细菌产生毒素的情况。14株嗜水气单胞菌中的5株以及15株温和气单胞菌中的4株产生了一种在免疫方面与霍乱肠毒素相关的因子。通过这些测定法以及热稳定性试验分析表明，该因子不同于溶血素以及在乳鼠试验中具有活性的毒素。
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