Parvez Z, Khan T, Moncada R
Invest Radiol. 1985 Jul;20(4):407-12. doi: 10.1097/00004424-198507000-00013.
A rat model was employed to investigate contrast media (CM) induced ultrastructural changes in the vascular endothelium. Ionic contrast materials such as Renografin-76 (diatrizoate meglumine diatrizoate sodium), MD-76 (diatrizoate meglumine diatrizoate sodium), and Angiovist (meglumine diatrizoate) were injected into the femoral vein of anesthetized male Wistar rats (240-260 g) and allowed to circulate. Control animals were similarly injected with equiosmolar sucrose and physiologic saline. The thorax was opened 15 minutes, 1 hour, and 4 hours postinjection and cardiac perfusion performed using Karnovsky's fixative; the thoracic aorta was then surgically removed, and processed for transmission electron microscopy. All CM produced shrinkage in cell cytoplasm and nuclear structures thereby causing distortions in cell morphology. In control tissues, however, no such ultrastructural damages were noted. Within 15 minutes of CM infusion, electron dense granules were seen on the luminal surface of endothelial cells, in pinocytotic vesicles, as well as in the gap junctions between cells. These observations indicate that contrast media intake occurs via vesicular transport, and through the cell junction.
采用大鼠模型研究造影剂（CM）诱导的血管内皮超微结构变化。将离子型造影剂如泛影葡胺-76（泛影葡胺和泛影酸钠）、MD-76（泛影葡胺和泛影酸钠）和安吉欧维斯（泛影葡胺）注入麻醉的雄性Wistar大鼠（240-260g）的股静脉并使其循环。对照动物同样注射等渗蔗糖和生理盐水。注射后15分钟、1小时和4小时打开胸腔，使用卡诺夫斯基固定液进行心脏灌注；然后手术切除胸主动脉，并进行透射电子显微镜检查。所有造影剂均导致细胞质和核结构收缩，从而引起细胞形态扭曲。然而，在对照组织中未观察到此类超微结构损伤。在注入造影剂后15分钟内，在内皮细胞的腔表面、吞饮小泡以及细胞间的缝隙连接中可见电子致密颗粒。这些观察结果表明造影剂通过囊泡转运和细胞连接进入细胞。
