Hughes J H, Gnau J M, Hilty M D, Chema S, Ottolenghi A C, Hamparian V V
J Med Microbiol. 1977 May;10(2):203-12. doi: 10.1099/00222615-10-2-203.
Immune electronmicroscopy (IEM) was used to identify human picornaviruses rapidly and to differentiate enteroviruses from rhinoviruses. Human sera, diluted 10- to 50-fold beyond the neutralisation endpoints for homologous virus, readily agglutinated C-type antigens of seven human picornaviruses. Human sera did not react by IEM with a control animal picornavirus. By IEM after acid treatment, differentiation of a human enterovirus from a human rhinovirus was possible. There was an excellent correlation between the results of IEM and immunodiffusion (ID) tests for the presence of antibody to human picornavirus group antigens. By ID, only one of 21 human sera reacted with one of six animal picornaviruses. Immune electronmicroscopy appears to be a sensitive and simple techinque for the detection of picornavirus C-type antigens, and may be useful for identifying viruses belonging to groups comprising many serotypes and sharing a common group antigen.
免疫电子显微镜技术（IEM）用于快速鉴定人微小核糖核酸病毒，并区分肠道病毒和鼻病毒。将人血清稀释至超过同源病毒中和终点10至50倍后，能轻易凝集7种人微小核糖核酸病毒的C型抗原。人血清通过IEM不与对照动物微小核糖核酸病毒发生反应。经过酸处理后通过IEM，可区分人肠道病毒和人鼻病毒。对于人微小核糖核酸病毒群抗原抗体的存在，IEM结果与免疫扩散（ID）试验结果之间存在极佳的相关性。通过ID检测，21份人血清中只有1份与6种动物微小核糖核酸病毒中的1种发生反应。免疫电子显微镜技术似乎是一种检测微小核糖核酸病毒C型抗原的灵敏且简便的技术，可能有助于鉴定属于包含多种血清型且共享共同群抗原的病毒群的病毒。
