Zabel M, Schäfer H
Histochemistry. 1985;83(1):77-80. doi: 10.1007/BF00495304.
Using pre- and post-embedding procedures, neuron-specific enolase and calcitonin were localized in rat thyroid parafollicular cells by light and electron microscopy. Peroxidase-antiperoxidase (PAP), biotin-avidin (ABC) and protein A--colloidal gold techniques were used. In paraffin sections neuron-specific enolase was demonstrated in all calcitonin-storing parafollicular cells in rats aging 1 to 180 days. The post-embedding procedure failed to detect neuron-specific enolase in ultrathin sections, but the enzyme could be demonstrated using a preembedding procedure. Neuron-specific enolase was localized exclusively within the cytosol of parafollicular cells, while calcitonin was localized within secretory granules applying either post- or pre-embedding incubation techniques.
采用包埋前和包埋后处理程序，通过光镜和电镜对大鼠甲状腺滤泡旁细胞中的神经元特异性烯醇化酶和降钙素进行定位。使用了过氧化物酶-抗过氧化物酶（PAP）、生物素-抗生物素蛋白（ABC）和蛋白A-胶体金技术。在石蜡切片中，在1至180日龄大鼠的所有储存降钙素的滤泡旁细胞中均检测到神经元特异性烯醇化酶。包埋后处理程序未能在超薄切片中检测到神经元特异性烯醇化酶，但使用包埋前处理程序可显示该酶。神经元特异性烯醇化酶仅定位在滤泡旁细胞的胞质溶胶内，而降钙素则通过包埋后或包埋前孵育技术定位在分泌颗粒内。
