Bradbury W C, Munroe D L
J Clin Microbiol. 1985 Sep;22(3):339-46. doi: 10.1128/jcm.22.3.339-346.1985.
Serologically defined strains of Campylobacter jejuni and Campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid DNA in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. Of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid DNA. A plasmid occurrence rate of 73.8% was obtained for C. coli from healthy pigs, contrasted by lower plasmid occurrence rates for C. coli from diarrheic pigs (30%) and from all diarrheic animals (21.4%). For C. jejuni, in contrast, only 13.6% of healthy cattle contained plasmid DNA, contrasted by a higher plasmid occurrence rate of 31.2% from diarrheic cattle. A high plasmid occurrence rate of 75.8% was observed for C. jejuni from healthy chickens. Campylobacter plasmids ranged in size from less than or equal to 1 to 86 megadaltons. Antibiotic susceptibility for 52 animal isolates (excluding chickens) indicated that most isolates were susceptible to kanamycin, erythromycin, gentamicin, tetracycline, and compound sulfonamide, whereas few were susceptible to bacitracin (19.2%); approximately half were susceptible to ampicillin (55.8%) and streptomycin (51.9%), and no isolates were susceptible to penicillin G. More isolates containing plasmids were resistant to ampicillin, tetracycline, and gentamicin than were isolates not carrying plasmids, there being a statistically significant difference for tetracycline and gentamicin, which suggested that these two antibiotics were probably plasmid mediated. The antibiotic susceptibility patterns of 21 chicken isolates of C. jejuni, by contrast, were different in that most were susceptible to ampicillin in addition to kanamycin, erythromycin, and gentamicin, whereas few wer susceptible to compound sulfonamide, streptomycin, and tetracycline in addition to penicillin G and bacitracin. A 30- or 39-megadalton plasmid, or both, common to many of the chicken isolates was usually associated with tetracycline resistance.
对来自健康和腹泻动物的空肠弯曲菌和结肠弯曲菌的血清学定义菌株进行了检查，以确定质粒DNA的存在情况及其与细菌宿主的抗生素敏感性和动物宿主健康状况的关系。在所有被调查的弯曲菌中，53%（200株中的116株）含有质粒DNA。健康猪源结肠弯曲菌的质粒出现率为73.8%，而腹泻猪源结肠弯曲菌（30%）和所有腹泻动物源结肠弯曲菌（21.4%）的质粒出现率较低。相比之下，空肠弯曲菌中，只有13.6%的健康牛含有质粒DNA，而腹泻牛的质粒出现率较高，为31.2%。健康鸡源空肠弯曲菌的质粒出现率高达75.8%。弯曲菌质粒大小范围从小于或等于1到86兆道尔顿。对52株动物分离株（不包括鸡）的抗生素敏感性检测表明，大多数分离株对卡那霉素、红霉素、庆大霉素、四环素和复方磺胺敏感，而对杆菌肽敏感的很少（19.2%）；约一半对氨苄西林（55.8%）和链霉素（51.9%）敏感，没有分离株对青霉素G敏感。与不含质粒的分离株相比，更多含质粒的分离株对氨苄西林、四环素和庆大霉素耐药，四环素和庆大霉素存在统计学显著差异，这表明这两种抗生素可能由质粒介导。相比之下，21株空肠弯曲菌鸡分离株的抗生素敏感性模式不同，大多数除对卡那霉素、红霉素和庆大霉素敏感外，还对氨苄西林敏感，而除对青霉素G和杆菌肽外，很少对复方磺胺、链霉素和四环素敏感。许多鸡分离株共有的一个30或39兆道尔顿的质粒，或两者都有，通常与四环素耐药性有关。
