McAnany J Jason, Park Jason C
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, 1855 W. Taylor St., MC/648, Chicago, IL, USA.
Department of Biomedical Engineering, University of Illinois at Chicago, 851 South Morgan St., Chicago, IL, 60607, USA.
Doc Ophthalmol. 2025 May 31. doi: 10.1007/s10633-025-10030-5.
To determine if harmonic components of the 30 Hz flicker ERG are useful for detecting neural dysfunction in diabetics who have mild or no non-proliferative diabetic retinopathy (NPDR).
Previously reported light-adapted flicker ERG data recorded from 20 diabetics who had no clinically-apparent retinopathy (NDR), 20 who had mild NPDR (MDR), and 20 non-diabetic controls were reanalyzed. From this dataset, the amplitude and phase of the 31.25 Hz flicker ERG fundamental and second harmonic were extracted. The 62.5 Hz flicker ERG fundamental was also extracted. Similar responses were also acquired prospectively from 10 controls, 5 NDR, and 5 MDR subjects, comprising a second dataset.
Analysis of variance indicated that both diabetic groups had normal amplitudes elicited by the 31.25 Hz stimulus (fundamental and second harmonic), whereas the 62.5 Hz amplitude was reduced significantly in both diabetic groups. This pattern was found in both the retrospective and prospective analyses.
The second harmonic of the 31.25 Hz flicker response (equivalent to 62.5 Hz) was normal in early-stage DR, whereas the response to 62.5 Hz flicker stimuli was abnormal. The second harmonic of the ISCEV standard 30 Hz flicker ERG does not appear to be a useful indicator of neural dysfunction in early DR.
确定30Hz闪烁视网膜电图（ERG）的谐波成分是否有助于检测患有轻度或无非增殖性糖尿病视网膜病变（NPDR）的糖尿病患者的神经功能障碍。
对先前报道的从20名无临床明显视网膜病变（NDR）的糖尿病患者、20名轻度NPDR（MDR）患者和20名非糖尿病对照者记录的明适应闪烁ERG数据进行重新分析。从该数据集中，提取31.25Hz闪烁ERG基波和二次谐波的振幅和相位。还提取了62.5Hz闪烁ERG基波。另外前瞻性地从10名对照者、5名NDR患者和5名MDR患者中获取了类似的反应，组成第二个数据集。
方差分析表明，两个糖尿病组由31.25Hz刺激（基波和二次谐波）诱发的振幅正常，而两个糖尿病组的62.5Hz振幅均显著降低。在回顾性和前瞻性分析中均发现了这种模式。
在早期糖尿病视网膜病变中，31.25Hz闪烁反应的二次谐波（相当于62.5Hz）正常，而对62.5Hz闪烁刺激的反应异常。国际临床视觉电生理学会（ISCEV）标准30Hz闪烁ERG的二次谐波似乎不是早期糖尿病视网膜病变中神经功能障碍的有用指标。
