Barreiro-Crespo Lourdes, Sanchez-Henao Andres, Gimeno-Monforte Sandra, Reverté Jaume, Campàs Mònica, Tsumuraya Takeshi, Diogène Jorge, Tunin-Ley Alina, Maillot Fanny, Flores Cintia, Fontanals Núria, Borrull Francesc, Turquet Jean, Rambla-Alegre Maria
IRTA, Marine and Continental Waters, Ctra. Poble Nou, km 5.5, 43540, La Ràpita, Spain; Universitat Rovira i Virgili, Department of Analytical Chemistry and Organic Chemistry, C/ Marcel·lí Domingo s/n, 43007, Tarragona, Spain.
IRTA, Marine and Continental Waters, Ctra. Poble Nou, km 5.5, 43540, La Ràpita, Spain; University Institute of Animal Health and Food Safety (IUSA), Ctra. Transmontaña S/N, 35413 Arucas, Spain.
Harmful Algae. 2025 Aug;147:102882. doi: 10.1016/j.hal.2025.102882. Epub 2025 May 5.
Several outbreaks of ciguatera poisoning have been recorded in La Réunion (SW Indian Ocean) since 1986. Toxicities and toxin profiles of fish from the Indian Ocean are poorly documented. In this work, a multidisciplinary study on the toxicity of fish from the Indian Ocean by neuro-2a cell-based assay was assessed, and the CTX-like compounds were evaluated by a magnetic bead-based immunoassay. The toxin profile is described by liquid chromatography coupled to tandem mass spectrometry and high-resolution mass spectrometry. The liver, muscle, and viscera of four fish specimens of three different species (one Lutjanus bohar, two Variola louti and one Carcharinus leucas) caught in the waters of La Réunion were analysed. One of the V. louti specimens was obtained after a CP incident. Their toxicity values ranged between 0.01 and 2.69 μg CTX1B equiv.·kg in flesh, 1.60 and 6.50 μg CTX1B equiv.·kg in liver and 0.07 and 4.77 μg CTX1B equiv.·kg in viscera. In general, liver and viscera showed higher concentrations of CTX-like compounds than flesh. Several potential CTX congeners were identified. LC-MS/MS confirmed the presence of CTX1B, 52-epi-54-deoxyCTX1B and 54-deoxyCTX1B in the flesh of the V. louti specimen involved in the CP incident. CTX1B was identified in the three tissues of the other V. louti individual and in the L. bohar. The C. leucas samples revealed the presence of a complex CTXs profile showing congeners of the CTX3C-group. The strategy used within this research work could be a valuable tool for future food safety monitoring in the Indian Ocean area.
自1986年以来，留尼汪岛（印度洋西南部）已记录了几起雪卡毒素中毒事件。关于印度洋鱼类的毒性和毒素谱的文献记载很少。在这项研究中，通过基于神经-2a细胞的分析方法对印度洋鱼类的毒性进行了多学科研究，并通过基于磁珠的免疫分析法对类CTX化合物进行了评估。毒素谱通过液相色谱-串联质谱和高分辨率质谱进行描述。对在留尼汪岛海域捕获的三种不同物种的四个鱼样本（一条波纹唇鱼、两条波纹裸胸鳝和一条公牛真鲨）的肝脏、肌肉和内脏进行了分析。其中一条波纹裸胸鳝样本是在一次雪卡毒素中毒事件后获得的。它们的毒性值在鱼肉中为0.01至2.69微克CTX1B当量·千克，肝脏中为1.60至6.50微克CTX1B当量·千克，内脏中为0.07至4.77微克CTX1B当量·千克。一般来说，肝脏和内脏中的类CTX化合物浓度高于鱼肉。鉴定出了几种潜在的CTX同系物。液相色谱-串联质谱证实，参与雪卡毒素中毒事件的波纹裸胸鳝样本的鱼肉中存在CTX1B、52-表-54-脱氧CTX1B和54-脱氧CTX1B。在另一条波纹裸胸鳝个体的三种组织以及波纹唇鱼中鉴定出了CTX1B。公牛真鲨样本显示出复杂的CTX谱，含有CTX3C组的同系物。本研究工作中使用的策略可能是未来印度洋地区食品安全监测的一个有价值的工具。
