Guo Yuqi, Zhang Xi, Xing Yanan, Liu Yuxuan, Liu Yixiao, Jia Liuya, Xu Lixin, He Gaosheng, Ouyang Jiting, Yan Xu
Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, People's Republic of China.
School of Physics, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
Sci Rep. 2025 May 31;15(1):19127. doi: 10.1038/s41598-025-03914-8.
The interaction of atmospheric pressure plasma (APP) with the liquids has been fully studied, and multiple biomedical applications of APP-activated liquids have also been verified. However, there is still no direct evidence regarding the interaction between APP treatment and serum. In the present study, the rat serum was treated with the helium APP device. Compared with APP-treated saline, the elevation of reactive oxygen species (ROS) levels in serum was significantly smaller, implying that an antioxidant system existed in serum. The proteomics suggested that differently expressed proteins were predominantly enriched in the glutathione metabolism pathway. Our results showed that APP treatment significantly increased the serum GSH level and GSH/GSSG ratio, suggesting the enhanced antioxidant capacity of the APP-treated serum. Furthermore, the antioxidant enzymes in APP-treated serum were examined. As a result, both the glutathione peroxidase (GPX) protein level and enzyme activity were up-regulated according to the parallel reaction monitoring (PRM) analysis and GPX enzyme activity analysis. Meanwhile, the activities of glutathione reductase (GR) and superoxide dismutase (SOD) enzymes were also enhanced after APP treatment, while the catalase (CAT) activity decreased. Meanwhile, the total antioxidant capacity (T-AOC) also increased, as confirmed by the evidence of the superior ROS scavenging by APP-treated serum. Our data highlight that APP treatment can enhance the antioxidant capacity of the serum, which is closely related to the up-regulated GSH level, GSH/GSSG ratio and antioxidant enzymes within serum after APP treatment.
大气压力等离子体（APP）与液体之间的相互作用已得到充分研究，APP激活液体的多种生物医学应用也已得到验证。然而，关于APP处理与血清之间的相互作用仍没有直接证据。在本研究中，用氦气APP装置处理大鼠血清。与APP处理的生理盐水相比，血清中活性氧（ROS）水平的升高明显较小，这意味着血清中存在抗氧化系统。蛋白质组学表明，差异表达的蛋白质主要富集在谷胱甘肽代谢途径中。我们的结果表明，APP处理显著提高了血清谷胱甘肽（GSH）水平和GSH/GSSG比值，表明APP处理的血清抗氧化能力增强。此外，还检测了APP处理血清中的抗氧化酶。结果，根据平行反应监测（PRM）分析和谷胱甘肽过氧化物酶（GPX）酶活性分析，GPX蛋白水平和酶活性均上调。同时，APP处理后谷胱甘肽还原酶（GR）和超氧化物歧化酶（SOD）的活性也增强，而过氧化氢酶（CAT）活性降低。同时，总抗氧化能力（T-AOC）也增加，这通过APP处理血清具有更强的ROS清除能力得到证实。我们的数据突出表明，APP处理可以增强血清的抗氧化能力，这与APP处理后血清中上调的GSH水平、GSH/GSSG比值和抗氧化酶密切相关。
