Knospe W H, Husseini S G, Adler S S, Reddi A H
Int J Cell Cloning. 1985 Sep;3(5):320-9. doi: 10.1002/stem.5530030505.
Implants of bone and tooth matrix powder were placed subcutaneously (s.c.) on intraperitoneal (i.p.) Mitex or Polyvic membranes. Implants were removed for histology after 1-24 weeks. Macrophages, fibroblasts, and vascular sinusoids infiltrated around bone and tooth matrix particles after one week. In the s.c. tooth matrix implants, a few sites of cartilage formation and ossification developed at two weeks, and by three weeks granulocytopoiesis and megakaryocytopoiesis developed adjacent to new bone; erythropoiesis was not observed. In s.c. bone matrix implants and in the i.p. artificial membranes coated with bone or tooth matrix powder, ossification or hematopoiesis was not observed. Small numbers of CFU-s, CFU-nm, BFU-e, and CFU-e appeared 10-20 days after s.c. implantation of tooth matrix; none were detected in s.c. bone matrix implants.
将骨和牙基质粉末植入物皮下（s.c.）放置在腹腔内（i.p.）的Mitex或Polyvic膜上。1至24周后取出植入物进行组织学检查。一周后，巨噬细胞、成纤维细胞和血管窦围绕骨和牙基质颗粒浸润。在皮下牙基质植入物中，两周时出现了一些软骨形成和骨化部位，到三周时，粒细胞生成和巨核细胞生成在新骨附近出现；未观察到红细胞生成。在皮下骨基质植入物以及腹腔内涂有骨或牙基质粉末的人工膜中，未观察到骨化或造血现象。皮下植入牙基质10至20天后出现少量的脾集落形成单位（CFU-s）、非红系集落形成单位（CFU-nm）、爆式红系集落形成单位（BFU-e）和红系集落形成单位（CFU-e）；皮下骨基质植入物中未检测到这些细胞。
