Meles Dewa Ketut, Mustofa Imam, Wurlina Wurlina, Rosyada Zulfi Nur Amrina, Khairullah Aswin Rafif, Rachmawati Kadek, Hidayah Astri Nur, Suwasanti Niluh, Mustofa Rheza Imawan, Akintunde Adeyinka Oye, Ahmad Riza Zainuddin, Ekawasti Fitrine, Riwu Katty Hendriana Priscilia, Riwu Audrey Gracelia
Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2025 Apr;15(4):1734-1746. doi: 10.5455/OVJ.2025.v15.i4.25. Epub 2025 Apr 30.
Nephrolithiasis is a kidney disorder caused by the formation of crystals through physiological and chemical processes in the body, with calcium oxalate being a major contributor. leaves are known for their antioxidant, diuretic, and anti-inflammatory properties and contain alkaloids, flavonoids, polyphenols, polysaccharides, saponins, phenolics, and tannins.
This study aimed to investigate the potential of leaf extract (MLE) as a calcium oxalate crystal inhibitor, its effect on reducing malondialdehyde (MDA) levels, enhancing superoxide dismutase (SOD) activity, and histopathological changes in kidneys.
Fifty male aged 12 weeks and weighing 202.43 ± 30.39 g were divided into five groups of 10. The groups included: normal control (C) group without treatment, the untreated ethylene glycol (EG)-induced group (T0), and the EG-induced groups treated with MLE at doses of 200 mg/kgbw (T1), 316 mg/kgbw (T2), and 500 mg/kgbw (T3) at 4-hour intervals after EG induction. EG and MLE were administered orally using a gastric tube for 21 days. The parameters observed included SOD and MDA activity, calcium oxalate crystals, and kidney histopathology.
MLE inhibited calcium oxalate crystal formation, reduced MDA levels, increased SOD activity, and prevented tubular epithelial cell degeneration, epithelial necrosis, and interstitial inflammation in the kidneys.
MLE demonstrated calcium oxalate crystal dissolution activity, antioxidant effects by reducing MDA content, enhanced SOD activity, and improved kidney conditions, thereby contributing to good health and well-being.
肾结石是一种由于体内生理和化学过程形成晶体而导致的肾脏疾病，草酸钙是主要成因。[植物名称]叶以其抗氧化、利尿和抗炎特性而闻名，含有生物碱、黄酮类化合物、多酚、多糖、皂苷、酚类和单宁。
本研究旨在探讨[植物名称]叶提取物（MLE）作为草酸钙晶体抑制剂的潜力，其对降低丙二醛（MDA）水平、增强超氧化物歧化酶（SOD）活性以及肾脏组织病理学变化的影响。
将50只12周龄、体重202.43±30.39 g的雄性[动物名称]分为五组，每组10只。分组包括：未处理的正常对照组（C）、未处理的乙二醇（EG）诱导组（T0）以及在EG诱导后每隔4小时分别用200 mg/kgbw（T1）、316 mg/kgbw（T2）和500 mg/kgbw（T3）剂量的MLE处理的EG诱导组。EG和MLE通过胃管口服给药21天。观察的参数包括SOD和MDA活性、草酸钙晶体以及肾脏组织病理学。
MLE抑制了草酸钙晶体的形成，降低了MDA水平，并增加了SOD活性，还预防了肾脏肾小管上皮细胞变性、上皮坏死和间质炎症。
MLE表现出草酸钙晶体溶解活性，通过降低MDA含量发挥抗氧化作用，增强了SOD活性，并改善了肾脏状况，从而有助于身体健康和幸福。
