摩苏尔市水样中[具体物质]的分离与分子检测。（原文中“from water samples in Mosul city”前缺少具体所指物质，这里根据语境补充了“[具体物质]”）

Isolation and molecular detection of from water samples in Mosul city.

作者信息

Al-Jarjary Senaa Abdullah Ali, Hasan Manal H, Sheet Omar Hashim

机构信息

Department of Biology, College of Science, University of Mosul, Mosul, Iraq.

Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.

出版信息

Open Vet J. 2025 Apr;15(4):1757-1764. doi: 10.5455/OVJ.2025.v15.i4.27. Epub 2025 Apr 30.

DOI:10.5455/OVJ.2025.v15.i4.27

PMID:40453845

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12124784/

Abstract

BACKGROUND

can be transferred via water- and food-borne routes, and it causes diseases; therefore, it is considered a major public health concern worldwide.

AIM

The objectives of this study were to detect in water using microscopic examination and molecular biology and to determine the relationship between in the current research and registered in GenBank based on the phylogenetic tree.

METHODS

Sixty-five water samples (35 house tanks and 30 tap water) were collected from Mosul city's left and right sides between September 2023 and March 2024.

RESULTS

The present study indicates that the prevalence of in water in Mosul City, based on microscopic examination, was 49.2%. The highest occurrence of in water was observed on the right side at 54.5%, while the lowest occurrence, at 43.8%, was observed on the left side. Furthermore, the occurrence of was 68.6% in house tank water samples was 68.6%, while in tap water samples, it was 26.7%. Furthermore, the PCR as revealed that was detected in 3.13% (1 of 32) of the water samples collected from Mosul. A novel strain of has been registered in the NCBI GenBank.

CONCLUSION

Proper hygiene during water sterilization and storage can help keep water uncontaminated for an extended period.

摘要

背景

可通过水传播和食物传播途径传播，并引发疾病；因此，它被认为是全球主要的公共卫生问题。

目的

本研究的目的是使用显微镜检查和分子生物学方法检测水中的[具体物质]，并基于系统发育树确定当前研究中的[具体物质]与GenBank中注册的[具体物质]之间的关系。

方法

2023年9月至2024年3月期间，从摩苏尔市左右两侧采集了65份水样（35份家庭水箱水样和30份自来水样）。

结果

本研究表明，基于显微镜检查，摩苏尔市水中[具体物质]的患病率为49.2%。右侧水中[具体物质]的发生率最高，为54.5%，而左侧最低，为43.8%。此外，家庭水箱水样中[具体物质]的发生率为68.6%，而自来水中为26.7%。此外，PCR检测显示，从摩苏尔采集的水样中有3.13%（32份中的1份）检测到[具体物质]。一种新型的[具体物质]菌株已在NCBI GenBank中注册。