Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Biomed Res Int. 2017;2017:4636420. doi: 10.1155/2017/4636420. Epub 2017 Nov 6.
Cryptosporidiosis and cyclosporiasis are caused by waterborne coccidian protozoan parasites of the genera and respectively. This study was conducted to detect and oocysts from environmental water abstracted by drinking water treatment plants and recreational activities in Sarawak, Malaysia. Water samples (12 each) were collected from Sungai Sarawak Kanan in Bau and Sungai Sarawak Kiri in Batu Kitang, respectively. In addition, 6 water samples each were collected from Ranchan Recreational Park and UNIMAS Lake at Universiti Malaysia Sarawak, Kota Samarahan, respectively. Water physicochemical parameters were also recorded. All samples were concentrated by the iron sulfate flocculation method followed by the sucrose floatation technique. and were detected by modified Ziehl-Neelsen technique. Correlation of the parasites distribution with water physicochemical parameters was analysed using bivariate Pearson correlation. Based on the 24 total samples of environmental water abstracted by drinking water treatment plants, all the samples (24/24; 100%) were positive with , and only 2 samples (2/24; 8.33%) were positive with . Based on the 12 total samples of water for recreational activities, 4 samples (4/12; 33%) were positive with , while 2 samples (2/12; 17%) were positive with . oocysts were negatively correlated with dissolved oxygen (DO).
隐孢子虫病和环孢子虫病是由水生球虫原生动物寄生虫引起的，分别属于 和 属。本研究旨在检测来自马来西亚砂拉越州的饮用水处理厂和娱乐活动采集的环境水中的 和 卵囊。从 Bau 的 Sarawak Kanan 河和 Batu Kitang 的 Sarawak Kiri 河分别采集了 12 个水样。此外，从兰昌娱乐公园和砂拉越大学 UNIMAS 湖分别采集了 6 个水样，砂拉越大学 UNIMAS 湖位于哥打三马拉汉。还记录了水样的理化参数。所有样品均采用硫酸铁絮凝法浓缩，然后采用蔗糖漂浮技术。采用改良的齐尔-尼尔森技术检测 和 。采用双变量 Pearson 相关分析寄生虫分布与水理化参数的相关性。根据从饮用水处理厂抽取的 24 个环境水样的检测结果，所有水样（24/24；100%）均呈阳性，检出 ，只有 2 个水样（2/24；8.33%）呈阳性，检出 。根据 12 个用于娱乐活动的水样的检测结果，有 4 个水样（4/12；33%）呈阳性，检出 ，有 2 个水样（2/12；17%）呈阳性，检出 。 卵囊与溶解氧（DO）呈负相关。
