Honardoost Maryam, Soleimanifar Fatemeh, Eslami Solat, Cheraghi Sara, Khamseh Mohammad Ebrahim, Darvish Maryam, Haddad Kashani Hamed
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
Cardio-Oncology Research Center, Rajaie Cardiovascular Research Institute, Iran University of Medical Sciences, Tehran, Iran.
Avicenna J Med Biotechnol. 2025 Apr-Jun;17(2):106-113. doi: 10.18502/ajmb.v17i2.18561.
Thyroid cancer is the most common type of cancer affecting the endocrine system. The main treatment approaches consist of surgical procedures and radioiodine therapy. Recently, there has been a heightened interest in investigating alternative treatment options, including probiotics, which could potentially minimize toxicity. Consequently, there is a growing imperative for research aimed at investigating the potential role of probiotics in the management of cancer.
The B-CPAP cell line was maintained in culture and tested with different dilutions of two bacterial strains. Toxicity evaluations were performed using the MTT assay to identify appropriate concentrations. mRNA was extracted and analyzed via real-time PCR to measure the expression levels of the , , and genes. Furthermore, changes in the cell cycle and the induction of apoptosis were examined using flow cytometry.
The supernatant derived from () - ATCC-393 and ()-GG demonstrated a significant inhibitory effect on the growth of B-CPAP cancer cells. The findings indicated that the combination produced a more pronounced anti-cancer effect by enhancing the expression of pro-apoptotic genes while reducing gene expression in B-CPAP cells.
The findings indicated a notable change in the expression of genes associated with apoptosis and modifications in the cell cycle. This implies that probiotics may enhance the efficacy of chemotherapy in treating thyroid cancer. In particular, and may play a beneficial role in the therapeutic process. Further research is required to investigate the direct impact of probiotics on thyroid function.
甲状腺癌是影响内分泌系统的最常见癌症类型。主要治疗方法包括外科手术和放射性碘治疗。最近，人们对研究替代治疗方案的兴趣日益浓厚，其中包括益生菌，其有可能将毒性降至最低。因此，开展旨在研究益生菌在癌症管理中潜在作用的研究变得越来越迫切。
将B-CPAP细胞系进行培养，并用两种细菌菌株的不同稀释液进行测试。使用MTT法进行毒性评估以确定合适的浓度。提取mRNA并通过实时PCR进行分析，以测量、和基因的表达水平。此外，使用流式细胞术检查细胞周期的变化和凋亡的诱导情况。
源自（）-ATCC-393和（）-GG的上清液对B-CPAP癌细胞的生长具有显著抑制作用。研究结果表明，该组合通过增强促凋亡基因的表达，同时降低B-CPAP细胞中的基因表达，产生了更明显的抗癌作用。
研究结果表明与凋亡相关的基因表达有显著变化，细胞周期也有改变。这意味着益生菌可能会增强化疗治疗甲状腺癌的疗效。特别是，和可能在治疗过程中发挥有益作用。需要进一步研究来调查益生菌对甲状腺功能的直接影响。
