Ma Jiaxiang, Yang Yu, Pei Ben, Mi Shengli, Xiong Zhuo, Ouyang Liliang
Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China.
Division of Advanced Manufacturing, Graduate school at Shenzhen, Tsinghua University, Shenzhen, 518055, China.
Adv Sci (Weinh). 2025 Aug;12(32):e02367. doi: 10.1002/advs.202502367. Epub 2025 Jun 4.
DNA data storage has emerged as a promising information storage technology by encoding information down to base molecules. However, it remains a challenge to structure the DNA data with ease of recording, retrieving, and reading. Here, a primer-disk-enabled hierarchical DNA data storage system is introduced, which allows for the multiple immobilizations of DNA molecules and the generation of corresponding QR codes for retrieving. The primer disk is pre-engineered to present multiple primers, on which encoded DNA molecules with complementary primers can be covalently immobilized on demand via solid-phase PCR. Each DNA file can be retrieved by inkjet printing a fluorescent QR code. A primer disk with up to 10 primers is used. The results show that different DNA files can be subsequently stored on the disk. One can have readily access to the index via fluorescent QR codes and decode information after sequent imaging, convention, and recognition. To this end, the recorded DNA files can be randomly read via solid-phase PCR with sufficient copies of collected DNA for up to 20 reads. Together, this work provides a new DNA data storage system with index and record-many-read-many features, paving the way for the practical use of DNA data storage.
通过将信息编码到碱基分子，DNA数据存储已成为一种很有前景的信息存储技术。然而，如何轻松地对DNA数据进行记录、检索和读取的结构化处理仍是一项挑战。在此，我们介绍了一种基于引物盘的分层DNA数据存储系统，该系统允许DNA分子的多次固定，并生成相应的二维码用于检索。引物盘经过预先设计，可呈现多个引物，通过固相PCR，带有互补引物的编码DNA分子可按需共价固定在引物盘上。每个DNA文件可通过喷墨打印荧光二维码进行检索。使用了一个带有多达10个引物的引物盘。结果表明，不同的DNA文件可随后存储在该盘上。通过荧光二维码可以方便地访问索引，并在后续成像、转换和识别后解码信息。为此，记录的DNA文件可通过固相PCR随机读取，收集到的DNA有足够的拷贝数，最多可读取20次。这项工作共同提供了一种具有索引和多次记录多次读取功能的新型DNA数据存储系统，为DNA数据存储的实际应用铺平了道路。
