Pernomiam Fabiana Car, Mandetta Amanda Rafaelly Honório, Ribeiro Caroline Diniz Pagani Vieira, Camargo Caroline Cristina Batista, Romano Iara Maria Freitas, Sobral Ana Paula Taboada, Mesquita-Ferrari Raquel Agnelli, Fernandes Kristianne Porta Santos, Horliana Anna Carolina Ratto Tempestini, Duran Cinthya Cosme Gutierrez, Motta Lara Jansiski, Bussadori Sandra Kalil
Postgraduate Programme in Rehabilitation Sciences, Universidade Nove de Julho - Campus Vergueiro, São Paulo, SP, Brazil.
Biophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, Brazil.
BMJ Open. 2025 Jun 4;15(6):e101725. doi: 10.1136/bmjopen-2025-101725.
Molar incisor hypomineralisation (MIH) is a qualitative developmental defect of the enamel with a complex, multifactorial nature and a significant genetic component. Individuals with MIH have a compromised stomatognathic system manifested by muscle hyperactivity under postural and dynamic conditions. However, there is a gap in knowledge on the specific functional abnormalities that these individuals experience. Early identification and intervention, with a focus on the prevention of orofacial dysfunctions and deviations in facial growth and development, are aspects of the utmost importance. Therefore, the aim of the proposed study is to perform a comparative analysis of orofacial functions with an emphasis on breathing and chewing patterns in individuals with and without MIH. The secondary objective is to assess whether dentin hypersensitivity and the severity of MIH lesions are associated with alterations in orofacial functions.
Assessments will be performed using the Nordic Orofacial Test-Screening (NOT-S). Descriptive analyses will characterise the sample. The Shapiro-Wilk test will assess normality. For normally distributed data, analysis of variance and Tukey's post hoc test will be used. For non-normal data, the Mann-Whitney U test will be applied. The χ2 test will analyse categorical variables and compare NOT-S domains between groups. Potential confounders (eg, age, sex, socioeconomic status) will be controlled through stratification or as covariates. Logistic and Poisson regressions will model associations for categorical and count-based outcomes, respectively. Statistical significance will be set at p<0.05.
This protocol has been approved by the Human Research Ethics Committee of Nove de Julho University (certificate number: 83969924.2.0000.5511; approval date: 22 November 2024). Participants will agree to take part in the study by signing an informed consent form. The findings will be published in a peer-reviewed journal. The collected data will be available on request.
NCT06692257.
磨牙症伴切牙矿化不全（MIH）是一种牙釉质的定性发育缺陷，具有复杂的多因素性质和显著的遗传成分。患有MIH的个体口颌系统受损，在姿势和动态条件下表现为肌肉活动亢进。然而，对于这些个体所经历的特定功能异常，目前仍存在知识空白。早期识别和干预，重点是预防口面部功能障碍以及面部生长发育偏差，是至关重要的方面。因此，本研究的目的是对有和没有MIH的个体的口面部功能进行比较分析，重点是呼吸和咀嚼模式。次要目标是评估牙本质过敏和MIH病变的严重程度是否与口面部功能的改变有关。
将使用北欧口面部测试筛查（NOT-S）进行评估。描述性分析将对样本进行特征描述。Shapiro-Wilk检验将评估正态性。对于正态分布的数据，将使用方差分析和Tukey事后检验。对于非正态数据，将应用Mann-Whitney U检验。χ2检验将分析分类变量并比较组间的NOT-S领域。潜在的混杂因素（如年龄、性别、社会经济地位）将通过分层或作为协变量进行控制。逻辑回归和泊松回归将分别对分类和基于计数的结果建立关联模型。统计学显著性设定为p<0.05。
本方案已获得七月九日大学人类研究伦理委员会的批准（证书编号：83969924.2.0000.5511；批准日期：2024年11月22日）。参与者将通过签署知情同意书同意参与本研究。研究结果将发表在同行评审期刊上。收集的数据将根据要求提供。
NCT06692257。
